Two Distinct Actin Networks Drive the Protrusion of Migrating Cells

By Aaron Ponti, M. Machacek, S. Gupton, C. Waterman-Storer, G. Danuser. Science, 2004

849 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cell Biology, Surfaces, Coatings and Films

View Paper: https://www.semanticscholar.org/paper/478144ae651f27cd1758a75721801c756685a9cd

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In Biomedical Engineering

In Cell Biology

In Surfaces, Coatings and Films

Erik H. W. Meijering

Citations in the corpus

Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner (2008, neutral): “Protrusions consist of two structurally and kinetically distinct actin network”
Actin dynamics, architecture, and mechanics in cell motility. (2014, background): “Transverse arcs mark out the region of the cell where actin flows slow down and where nascent cell-substrate contacts mature into stable focal adhesion structures (FIGURE 5, iii); however, transverse arcs are not believed to contact focal adhesions (5, 65, 247).”
Environmental sensing through focal adhesions (2009, background): “As a rule, the formation of the focal complexes occurs underneath the lamellipodia”
Spatiotemporal feedback between actomyosin and focal-adhesion systems optimizes rapid cell migration. (2006, methodology): “F-Actin Dynamics in the Lamellipodium We next used qFSM analysis software to produce spatiotemporal maps of the velocity of F-actin flow (kinematics) and the organization of F-actin assembly/disassembly rates (kinetics) (Ponti et al., 2004).”
The Open Microscopy Environment (OME) Data Model and XML file: open tools for informatics and quantitative analysis in biological imaging (2005, methodology): “Numerical analytic methods extract information from quantitative image data that cannot be gleaned by simple inspection [5-7].”
Regulation of actin assembly associated with protrusion and adhesion in cell migration. (2008, background): “Fast-moving and short-lived speckles defined the lamellipodium, and slow-moving and long-lived speckles defined the lamella (88, 167).”
Life at the leading edge. (2011, background): “Behind the highly dynamic lamellipodium is a more stable region, called the lamella, which contributes to cell migration by coupling the actin network to myosin II-mediated contractility and substrate adhesion (Ponti et al., 2004).”
The many faces of actin: matching assembly factors with cellular structures (2007, background): “; the second was the observation that two distinct actin-based networks constitute the lamellipodium and lamellu”
Analysis of the myosinII-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation (2011, background): “The composition of focal adhesions in myosin-II-inhibited cells probably reflects that of nascent adhesions that form and turn over rapidly during lamellipodial protrusion in the absence of local myosin II activit”
Directing cell motions on micropatterned ratchets (2009, background): “Fluorescent imaging suggests that this breaking of spatial symmetry is due to the reorganization of actin bundles, which, in turn, determines the direction of a cell’s lamellipodial protrusio”
Cell adhesion: integrating cytoskeletal dynamics and cellular tension (2010, background): “The initial step in the migration cycle, protrusion of a leading edge, is driven by actin polymerization in filaments organized into two distinct zones, the lamellipodium and the lamellu”
Physics of adherent cells (2013, background): “This then leads to flow of the actin in the direction opposite to treadmilling, that is away from the cell membrane (retrograde flow), as can be measured with speckle fluorescence microscopy (Ponti et al., 2004).”
Tracking in cell and developmental biology. (2009, background): “Computerized analysis of fluorescent speckle microscopy (FSM) movies of migrating cells have revealed that the protrusive behavior of the plasma membrane at the leading edge during migration is mediated by two spatially colocalized but kine(ma)tically and molecularly distinct actin networks related ”
Actin stress fibers – assembly, dynamics and biological roles (2012, background): “However, several recent studies provide evidence that, in migrating cells, the lamellum corresponds to the transverse arc network, as both structures are composed of condensed actin bundles and undergo retrograde flow towards the cell center (Ponti et al., 2004; Hotulainen and Lappalainen, 2006).”