Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner
By Colin K. Choi, M. Vicente-Manzanares, Jessica Zareno, Leanna A. Whitmore, A. Mogilner, A. Horwitz. Nature Cell Biology, 2008
664 citations recorded by the source corpus.
Using two-colour imaging and high resolution TIRF microscopy, we investigated the assembly and maturation of nascent adhesions in migrating cells. We show that nascent adhesions assemble and are stable within the lamellipodium. The assembly is independent of myosin II but its rate is proportional to the protrusion rate and requires actin polymerization. At the lamellipodium back, the nascent adhesions either disassemble or mature through growth and elongation. Maturation occurs along an α-actinin–actin template that elongates centripetally from nascent adhesions. α-Actinin mediates the formation of the template and organization of adhesions associated with actin filaments, suggesting that actin crosslinking has a major role in this process. Adhesion maturation also requires myosin II. Rescue of a myosin IIA knockdown with an actin-bound but motor-inhibited mutant of myosin IIA shows that the actin crosslinking function of myosin II mediates initial adhesion maturation. From these studies, we have developed a model for adhesion assembly that clarifies the relative contributions of myosin II and actin polymerization and organization.
Subfields: Cardiology and Cardiovascular Medicine, Cell Biology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/783a83b8a2232f060544cf553a25ae381d8a9f9c
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Citations in the corpus
- Genetic and cell biological analysis of integrin outside-in signaling. (2009, background): “Despite its ability to directly bind b integrins (Otey et al. 1990; Pavalko and LaRoche 1993), it is more likely that a-actinin operates as a close partner of actin in modulating the strength and stability of the integrin–actin linkage (Brown et al. 2006; Choi et al. 2008).”
- Mechanical Integration of Actin and Adhesion Dynamics in Cell Migration (2010, background): “Adhesion of a protruding cell edge is initiated by nascent adhesions, smaller than the ∼0.25 μm resolution of the light microscope, that assemble concurrent with lamellipodial protrusion (Choi et al. 2008).”
- The Actin Cytoskeleton and Actin-Based Motility (2018, background): “The forces required to form focal complexes are fairly small and can be produced by contraction of actin–myosin-II networks or derived from actin filament cross-linking (Choi et al. 2008) or a drag from actin retrograde flow (Alexandrova et al. 2008; Gardel et al. 2008).”
- Extracellular matrix and cell signalling: the dynamic cooperation of integrin, proteoglycan and growth factor receptor. (2011, neutral): “2007, Choi et al .”