A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegans

By S. W. Knight, B. Bass. Science, 2001

952 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/439bf77321d4d6c04efa3d060b4af7757ad8978f

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MicroRNA genes are transcribed by RNA polymerase II (2004, background): “Pre-miRNA is subsequently cleaved by cytoplasmic RNase III Dicer (Bernstein et al, 2001; Grishok et al, 2001; Hutvagner et al, 2001; Ketting et al, 2001; Knight and Bass, 2001) into B22-nt miRNA duplex.”
Fragile X-related protein and VIG associate with the RNA interference machinery. (2002, background): “Recent studies of Dicer function (Grishok et al. 2001; Hutvagner et al. 2001; Ketting et al. 2001; Knight and Bass 2001) and of Argonaute family members (Grishok et al. 2001) in C. elegans have revealed that endogenously encoded small hairpin RNAs (generically, miRNAs) regulate the expression of…”
The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. (2002, background): “elegans have been shown to interact, genetically or biochemically, with small RNAs, now known generically as microRNAs or miRNAs (Grishok et al. 2001; Hutvagner et al. 2001; Ketting et al. 2001; Knight and Bass 2001; Hutvagner and Zamore 2002; Mourelatos et al. 2002).”
miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. (2002, background): “Although these pathways are distinct, both require cleavage of double-stranded RNA (dsRNA) precursors by the nuclease Dicer to generate siRNAs or stRNAs (Bernstein et al. 2001; Grishok et al. 2001; Hutvagner et al. 2001; Ketting et al. 2001; Knight and Bass 2001).”
Human embryonic stem cells express a unique set of microRNAs. (2004, background): “Following this initial processing, pre-miRNAs get exported to the cytoplasm by Exportin-5 (Lund et al., 2003; Yi et al., 2003) and are subject to a second processing to generate the final product of approximately 22 nt mature miRNAs by another RNase III type protein Dicer (Grishok et al., 2001; Hutv”
Endogenous and Silencing-Associated Small RNAs in Plants Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.003210. (2002, background): “Both stRNAs and siRNAs arise by cleavage of precursor molecules containing exclusively or extensive duplex secondary structure by an RNaseIII-like enzyme (Bernstein et al., 2001; Hutvagner et al., 2001; Ketting et al., 2001; Knight and Bass, 2001), which in Drosophila was termed Dicer.”
MicroRNA functions in animal development and human disease (2005, background): “Suggestions for a role of microRNAs in the germline initially came from the analysis of Dicer loss-of-function mutants in C. elegans (Knight and Bass, 2002).”
Perspective: machines for RNAi. (2005, background): “These 21–25-nt double-stranded RNAs have a characteristic structure that reflects their production by the multidomain ribonuclease III (RNase III) enzyme, Dicer (Bernstein et al. 2001; Elbashir et al. 2001a,b; Ketting et al. 2001; Knight and Bass 2001; Xie et al. 2004).”