Identification of white adipocyte progenitor cells in vivo.

By Matthew S. Rodeheffer, Kıvanç Birsoy, J. Friedman. Cell, 2008

928 citations recorded by the source corpus.

The increased white adipose tissue (WAT) mass associated with obesity is the result of both hyperplasia and hypertrophy of adipocytes. However, the mechanisms controlling adipocyte number are unknown in part because the identity of the physiological adipocyte progenitor cells has not been defined in vivo. In this report, we employ a variety of approaches, including a noninvasive assay for following fat mass reconstitution in vivo, to identify a subpopulation of early adipocyte progenitor cells (Lin(-):CD29(+):CD34(+):Sca-1(+):CD24(+)) resident in adult WAT. When injected into the residual fat pads of A-Zip lipodystrophic mice, these cells reconstitute a normal WAT depot and rescue the diabetic phenotype that develops in these animals. This report provides the identification of an undifferentiated adipocyte precursor subpopulation resident within the adipose tissue stroma that is capable of proliferating and differentiating into an adipose depot in vivo.

Subfields: Epidemiology, Genetics, Physiology

View Paper: https://www.semanticscholar.org/paper/429a07e4f2995c5d23222e2b6633e7460420b94f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Epidemiology

In Genetics

In Physiology

Citations in the corpus

Satellite cells and the muscle stem cell niche. (2013, background): “In contrast, the minor population of CD45 /Sca-1 satellite cells can differentiate into both fibroblasts and adipocytes in culture, a similarity that is shared with CD45 / Sca-1 mesenchymal stem cells found in multiple tissues (316, 401, 417, 441, 509, 519).”
What We Talk About When We Talk About Fat (2014, neutral): “Both CD24 andCD24 cells could be converted to adipocytes in a dish, but only the former could reconstitute a functional fat pad when transplanted into a lipodystrophic mouse, and only when placed in an appropriate microenvironment (Rodeheffer et al., 2008).”
Adipose Tissue Remodeling: Its Role in Energy Metabolism and Metabolic Disorders (2016, background): “In mice, more specific subpopulation of adipogenic precursor cells have been characterized, including highly proadipogenic subpopulations of CD45–, CD31–, Ter119–, CD29+, CD34+, Sca-1+, and CD24+ cells (110).”
Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle (2010, methodology): “Adipocyte progenitor cells have been previously identified in white adipose tissue using a FACS-based cell isolation metho”
Adipocyte lineage cells contribute to the skin stem cell niche to drive hair cycling (2011, methodology): “Adipocyte precursor cell purification was performed as described (Rodeheffer et al., 2008).”
Beige Adipocytes are a Distinct Type of Thermogenic Fat Cell in Mouse and Human (2012, background): “presence of various environmental cues (Joe et al., 2009, 2010; Lee et al., 2012; Rodeheffer et al., 2008; Schulz et al., 2011; Uezumi et al., 2010).”
Hypertrophy and/or Hyperplasia: Dynamics of Adipose Tissue Growth (2009, background): “Recruitment of both types of progenitors has been shown to be stimulated by high-fat diet [41,43].”
Muscle injury activates resident fibro/adipogenic progenitors that facilitate myogenesis (2010, background): “Adipogenic progenitors expressing identical surface markers were recently isolated from white adipose depot”
Forming functional fat: a growing understanding of adipocyte differentiation (2011, background): “Technological advances in flow cytometry, the generation of transgenic animals and the identification of stem cell surface markers in other tissues have made it possible to isolate the subpopulation of fibroblasts in the stromal vascular fraction (SVF) of WAT that has adipogenic potentia”
In vivo identification of bipotential adipocyte progenitors recruited by β3-adrenoceptor activation and high fat feeding (2012, positive): “Several groups have isolated distinct cell populations from adipose tissues and muscle that have the potential to form white or brown adipocytes in vitro and in vivo, following transplantation (Joe et al., 2010; Petrovic et al., 2010; Rodeheffer et al., 2008; Schulz et al., 2011; Uezumi et al., 2010”
Adaptive thermogenesis in adipocytes: is beige the new brown? (2013, background): “Subpopulations of adipose progenitors have been isolated using different combinations of cell surface markers from enzyme-digested adipose tissue and skeletal muscle (Rodeheffer et al. 2008; Joe et al. 2010; Uezumi et al. 2010).”
Adipose tissue plasticity in health and disease (2022, background): “A ground-breaking study in 2008 utilized candidate cell surface markers to prospectively isolate and characterize APCs in WAT (Rodeheffer et al., 2008).”
Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration (2017, methodology): “Moreover, gene expression patterns of in-vitro-differentiated progenitors from bone resembled most closely those derived from iWAT, with similar adipogenic differentiation capacity and expression of general adipogenic genes Peroxisome proliferator-activated receptor-g (Pparg) and CCAAT/enhancer-bind”
Obesity as a Major Risk Factor for Cancer (2013, background): “It has been proposed that evolving bidirectional crosstalk is functional between breast cancer cells and tumour-surrounding adipocytes, and that the tumour-modified adipocytes are actively involved in tumour progression [101].”