In vivo identification of bipotential adipocyte progenitors recruited by β3-adrenoceptor activation and high fat feeding
By Yun-Hee Lee, Anelia P. Petkova, E. Mottillo, J. Granneman. Cell Metabolism, 2012
649 citations recorded by the source corpus.
Summary Nutritional and pharmacological stimuli can dramatically alter the cellular phenotypes in white adipose tissue (WAT). Utilizing genetic lineage tracing techniques, we demonstrate that brown adipocytes (BA) that are induced by β3-adrenergic receptor activation in abdominal WAT arise from the proliferation and differentiation of cells expressing platelet-derived growth factor receptor alpha (PDGFRα), CD34 and Sca1 (PDGFRα+ cells). PDGFRα+ cells have a unique morphology in which extended processes contact multiple cells in the tissue microenvironment. Surprisingly, these cells also give rise to white adipocytes (WA) that can comprise up to 25% of total fat cells in abdominal fat pads following 8 weeks of high fat feeding. Isolated PDGFRα+ cells differentiated into both BA and white adipocytes (WA) in vitro, and generated WA after transplantation in vivo. The identification of PDGFRα+ cells as bipotential adipocyte progenitors will enable further investigation of mechanisms that promote therapeutic cellular remodeling in adult WAT.
Subfields: Epidemiology, Nutrition and Dietetics, Physiology
View Paper: https://www.semanticscholar.org/paper/7c63b8d08f116b56d9a00df887dad60e9a262cba
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Citations in the corpus
- Brown and beige fat in humans: thermogenic adipocytes that control energy and glucose homeostasis. (2015, positive): “A large part of the newly formed beige adipocytes in subcutaneous WAT is negative for BrdU or Ki67, such that mitotic proliferation is not required for the cold-induced beige adipocyte differentiation (37, 41, 42).”
- Beige Adipocytes are a Distinct Type of Thermogenic Fat Cell in Mouse and Human (2012, background): “Notably, Sca-1+/CD45-/Mac1- (referred as ScaPCs; Schulz et al., 2011) and PDGFRa+/CD34+/Sca-1+ (referred as PDGFRa+ cells; Lee et al., 2012) have been suggested to have brown adipogenic potential.”
- A switch from white to brown fat increases energy expenditure in cancer-associated cachexia. (2014, methodology): “To better characterize the identity of proliferating cells in WAT of cachectic mice, we performed costaining experiments with established markers of adipocyte progenitors (Lee et al., 2012; Tang et al., 2011) and identified proliferating PDGFRapositive cells, but not PDGFRb or PPARg cells (Figure S3”
- Brown and beige fat: Physiological roles beyond heat-generation (2015, background): “For example, the epididymal WAT of mice, a depot that is much less prone to beige-ing, contains bipotent adipogenic precursor cells that can give rise to both white and beige (UCP1+) adipocytes, depending on environmental conditions (Lee et al., 2012).”
- Adipocyte Inflammation is Essential for Healthy Adipose Tissue Expansion and Remodeling (2014, background): “There is also evidence for increased adipogenesis in GWAT in response to chronic b3-AR-agonist treatment (Lee et al., 2012; Wang et al., 2013).”
- Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells (2012, background): “A recent study has shown that PDGFRa-positive bipotent progenitors in the abdominal WAT give rise to brown adipocytes in response to beta-adrenergic stimulation in vivo [18].”
- Adaptive thermogenesis in adipocytes: is beige the new brown? (2013, background): “A bipotential adipogenic subpopulation (PDGFRa+:Sca-1+:CD34+; so-called PDGFRa+ cells) has recently been proposed (Lee et al. 2012).”