Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

By D. Klionsky, H. Abeliovich, P. Agostinis, D. Agrawal, G. Aliev, D. Askew, M. Baba, Eric H. Baehrecke, B. Bahr, Andrea Ballabio and 225 more. Autophagy, 2008

2,657 citations recorded by the source corpus.

Subfields: Cell Biology, Epidemiology

View Paper: https://www.semanticscholar.org/paper/410bdf34d35dc10ece102c2a6511c12ce52e9217

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Citations in the corpus

Autophagy as a target for anticancer therapy (2011, background): “In addition to, or as an alternative to, direct LC3 detection, a green fluorescent protein (GFP)-LC3 construct can be measured”
Essential regulation of cell bioenergetics by constitutive InsP3 receptor Ca2+ transfer to mitochondria. (2010, positive): “In addition, presence of LC3-GFP puncta associated with early AV formation (Klionsky et al., 2008) was higher in KO (18% ± 4”
A vesicular transport pathway shuttles cargo from mitochondria to lysosomes. (2012, positive): “also treated H1299 cells stably expressing GFP-LC3 [12] with GO for 2 or 4 hr and probed for the activation of LC3 by the appearance of the cleaved GFP protein [13].”
Selective autophagy mediated by autophagic adapter proteins (2011, background): “, LC3-II) is tightly bound to the autophagosomal membranes and serves as an autophagic marker protein.(14,16,18,19) The conjugation of ATG8 to PE is essential for the hemifusion of lipid membranes and is suggested to drive the expansion of the autophagosome.”
Classification of cell death (2005, positive): “A very comprehensive description of the assays for monitoring autophagy in higher eukaryotes and a set of guidelines for their interpretation has been recently provided by Klionsky et al.38 One technique commonly employed to detect autophagy relies on the redistribution of GFP-LC3 fusion proteins in”
Dynamics and diversity in autophagy mechanisms: lessons from yeast (2009, background): “These features of Atg8 allow us to use this protein and its homologues to trace progression of autophagy in various organism”
Consensus guidelines for the detection of immunogenic cell death (2014, background): “This can be achieved by a wide panel of techniques, whose detailed discussion goes largely beyond the scope of this set of recommendations.(15,270,271) This said, one of the most convenient approaches to obtain insights into the autophagic response of cells exposed to homeostatic perturbations relie”
A role for ubiquitin in selective autophagy. (2009, methodology): “…potential (Ichimura et al., 2008; Pankiv et al., 2007). p62 is an LC3 interaction partner in vivo (Komatsu et al., 2007; Pankiv et al., 2007) and is constantly degraded by autophagy (Bjorkoy et al., 2005), establishing p62 as a useful marker for autophagic vesicle turnover (Klionsky et al., 2008).”
Nod1 and Nod2 direct autophagy by recruiting ATG16L1 to the plasma membrane at the site of bacterial entry (2010, background): “Notably, the greater number of GFP-LC3 dots was due to the induction of autophagy rather than to the inhibition of autophagosome maturation, as inhibitors of LC3 turnover, bafilomycin A1 and chloroquin”
Autophagy Induction and Autophagosome Clearance in Neurons: Relationship to Autophagic Pathology in Alzheimer's Disease (2008, background): “In macroautophagy, hereafter referred to as autophagy, regional sequestration of cytoplasm within an enveloping double membrane structure, creates a vacuole, termed the autophagosome (De Duve and Wattiaux, 1966; Klionsky et al., 2008).”