The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403.
By A. Bolotin, P. Wincker, S. Mauger, O. Jaillon, K. Malarme, J. Weissenbach, S. Ehrlich, A. Sorokin. Genome Research, 2001
1,134 citations recorded by the source corpus.
Lactococcus lactis is a nonpathogenic AT-rich gram-positive bacterium closely related to the genus Streptococcus and is the most commonly used cheese starter. It is also the best-characterized lactic acid bacterium. We sequenced the genome of the laboratory strain IL1403, using a novel two-step strategy that comprises diagnostic sequencing of the entire genome and a shotgun polishing step. The genome contains 2,365,589 base pairs and encodes 2310 proteins, including 293 protein-coding genes belonging to six prophages and 43 insertion sequence (IS) elements. Nonrandom distribution of IS elements indicates that the chromosome of the sequenced strain may be a product of recent recombination between two closely related genomes. A complete set of late competence genes is present, indicating the ability of L. lactis to undergo DNA transformation. Genomic sequence revealed new possibilities for fermentation pathways and for aerobic respiration. It also indicated a horizontal transfer of genetic information from Lactococcus to gram-negative enteric bacteria of Salmonella-Escherichia group.
Subfields: Ecology, Food Science, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/405f03a7a81f9d1719e24250b0deb67132c70389
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Citations in the corpus
- Phylogeny of the bacterial superfamily of Crp-Fnr transcription regulators: exploiting the metabolic spectrum by controlling alternative gene programs. (2003, neutral): “lactis has been completed [83].”
- Identification, Cloning, and Characterization of a Lactococcus lactis Branched-Chain α-Keto Acid Decarboxylase Involved in Flavor Formation (2005, neutral): “lactis IL1403 (8) a putative (partial) indole-3pyruvate decarboxylase gene and a 2-oxoglutarate decarboxylase gene have been identified but pyruvate decarboxylase and branched-chain keto acid decarboxylase genes are absent.”
- Prophages and bacterial genomics: what have we learned so far? (2003, background): “subtilis, Clostridium acetobutylicum, Clostridium perfringens, Clostridium tetani, Lactococcus lactis, Listeria innocua, Listeria monocytogenes, Staphylococcus aureus and Streptococcus pyogenes strains all carry multiple, easily recognizable and, in many cases, largely intact prophages (Kunst et al.”
- Global control of sugar metabolism: a gram-positive solution. (2002, background): “This is indicated by the presence of fifteen sugar-specific PTS permeases in B. subtilis ,n ine inLactococcus lactis and eight in Streptococcus pneumoniae (Reizer et al. 1999; Bolotin et al. 2001; Hoskins et al. 2001).”
- 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis (2005, background): “lactis is elucidated and largely publicly available (Bolotin et al. 2001; Klaenhammer et al. 2002).”
- Lactic acid bacteria: their applications in foods (2018, positive): “lactis IL1403) was sequenced and published [20].”
- The complex microbiota of raw milk. (2013, background): “Forty-three insertion elements were identified, the distribution of which suggests that recent recombination between two closely related genomes may have occurred (Bolotin et al., 2001).”
- Stress responses in lactic acid bacteria (2002, background): “lactis IL1403 revealed the presence of all genes necessary for aerobic respiration, as well as the genes involved in the late steps of heme synthesis (Bolotin et al. 2001).”