Patched acts catalytically to suppress the activity of Smoothened

By Jussi Taipale, M. K. Cooper, T. Maiti, P. Beachy. Nature, 2002

867 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/3431f8cf7bb638bd03af60e39f07049128c89e54

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

Communicating with Hedgehogs (2005, background): “PTC is a multipass transmembrane protein with similarity to the resistance-nodulation division (RND) family of prokaryotic permease”
Hedgehog Signaling in Development and Cancer (2008, neutral): “It is homologous to the RND family of prokaryotic proton-driven transporters, and several conserved features of RND-like transporters are essential for catalytic Ptc function in Smo inhibition, raising the interesting possibility that Ptc transports an endogenous small molecule Smo agonist or antago”
Wnts and Hedgehogs: lipid-modified proteins and similarities in signaling mechanisms at the cell surface (2003, background): “…requires not only a functional Fz, but also the presence of a long, single-pass transmembrane molecule of the Lrp (LDL receptor-related protein) family, which is encoded by arrow in Drosophila (Wehrli et al., 2000) and by Lrp5 or Lrp6 in vertebrates (Pinson et al., 2000; Tamai et al., 2000).”
Genome-wide analysis of ETS-family DNA-binding in vitro and in vivo (2010, neutral): “…(with exception of ETS domains in N- or C-terminal regions) were cloned into pMAGIC1 (Li and Elledge, 2005) or directly to pGEN expression vector (Taipale et al, 2002) from Megaman cDNA library (Stratagene) and from mouse-pooled cDNAs (mouse 12.5 days embryonic and fetal brain cDNA library; a…”
Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened. (2002, background): “No analogous protein interactions have been associated with Smo activation, and removal of the Smo CRD does not appear to significantly alter Smo function or its suppression by Ptch (Taipale et al. 2002).”
Mechanisms and functions of Hedgehog signalling across the metazoa (2011, background): “were refuted by the demonstration that PTC inhibits SMO non-stoichiometricall”
Mechanistic insights into the generation and transduction of Hedgehog Signaling (2020, background): “PTCH1 catalytically controls the activity of SMO without direct interaction [66]; PTCH1 may transport some small molecules, changing their concentrations in the cell membrane.”
Hedgehog: functions and mechanisms. (2008, background): “However, the fact that increasing the level of Ptc protein decreases cellular responsiveness to Hh (see Bailey et al. 2002; Taipale et al. 2002) indicates that it is the absolute amount of unliganded Ptc in a cell that controls pathway activity.”
Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours (2003, background): “Mutations that activate the Hh pathway include those that impair the ability of the transporter-like Hh recepto”