Stereotyped Odor-Evoked Activity in the Mushroom Body of Drosophila Revealed by Green Fluorescent Protein-Based Ca2+ Imaging

By Yalin Wang, Huifu Guo, T. Pologruto, F. Hannan, I. Hakker, Karel Svoboda, Yi Zhong. 2004

208 citations recorded by the source corpus.

To study the representation of olfactory information in higher brain centers, we expressed a green fluorescent protein-based Ca2+ sensor, G-CaMP, in the Drosophila mushroom body (MB). Using two-photon microscopy, we imaged odor-evoked G-CaMP fluorescence transients in MB neurons [Kenyon cells (KCs)] with single-cell resolution. Odors produced large fluorescence transients in a subset of KC somata and in restricted regions of the calyx, the neuropil of the MB. In different KCs, odor-evoked fluorescence transients showed diverse changes with odor concentration: in some KCs, fluorescence transients were evoked by an odor at concentrations spanning several orders of magnitude, whereas in others only at a narrow concentration range. Different odors produced fluorescence transients in different subsets of KCs. The spatial distributions of KCs showing fluorescence transients evoked by a given odor were similar across individuals. For some odors, individual KCs with fluorescence transients evoked by a particular odor could be found in similar locations in different flies with spatial precisions on the order of the size of KC somata. These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB.

Subfields: Cellular and Molecular Neuroscience, Nutrition and Dietetics, Sensory Systems

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Citations in the corpus

Re-evaluating circuit mechanisms underlying pattern separation (2019, positive): “Considerable evidence has now accumulated that the mushroom body employs ultrasparse coding, as studies using a variety of methods, including whole-cell recordings, tetrode recordings, and calcium imaging, all found that only around 5-10% of Kenyon cells were active upon odour presentation (Figure 3”
Testing odor response stereotypy in the Drosophila mushroom body. (2008, background): “Recent calcium imaging studies on KCs in flies also suggest broad stereotypy (Wang et al., 2004).”
Two pairs of mushroom body efferent neurons are required for appetitive long-term memory retrieval in Drosophila. (2013, background): “, 2008), where the specificity of memory toward a given odor is conferred by the sparse representation of olfactory stimuli in the KCs (Wang et al., 2004; Turner et al., 2008; Honegger et al., 2011), whereas an altered—in this case increased—response in the restricted number of output neurons is suf”
Three Dopamine Pathways Induce Aversive Odor Memories with Different Stability (2012, background): “represented by a small subset of Kenyon cells [10,11,12], which are major intrinsic neurons of the MB and categorized into the a/ b, a’/b’ and c neurons (Figure 1A) [13].”
Drosophila α/β Mushroom Body Neurons Form a Branch-Specific, Long-Term Cellular Memory Trace after Spaced Olfactory Conditioning (2006, unknown): “…Uas transgenes containing Drosophila the Creb2-b repressor coding region (Uas-dCreb2-b, see below) or G-CaMP (Uas-G-CaMP, Wang et al., 2003; Wang et al., 2004) were employed along with the c739-Gal4 driver (c739-Gal4 flies were from K. Kaiser, Division of Molecular Genetics, University of…”
A map of olfactory representation in the Drosophila mushroom body. (2007, unknown): “, 2004) and the MB calyx (Wang et al., 2004), but analysis of topographic connectivity between PNs and KCs in the MB calyx has been hampered by a lack of optical resolution and anatomical landmarks.”
Cellular-resolution population imaging reveals robust sparse coding in the Drosophila Mushroom Body (2011, background): “3 (Wang et al., 2004) and suggests that this indicator has the sensitivity to detect most KC activity.”
Divisive normalization in olfactory population codes (2010, background): “Many KCs are selective for a particular stimulus, and KCs tend to respond in a binary fashion, firing either zero spikes or just a few spikes (Stopfer et al., 2003; Wang et al., 2004; Turner et al., 2007).”
Comprehensive Maps of Drosophila Higher Olfactory Centers: Spatially Segregated Fruit and Pheromone Representation (2007, unknown): “This is likely to explain the observations of Wang et al. (2004) that certain odors can evoke spatially stereotyped activity in MB neurons.”
Olfactory information processing in Drosophila. (2009, unknown): “Later experiments imaging cell bodies observed calcium increases in only 2% of the Kenyon cell population [94].”
Are mushroom bodies cerebellum-like structures? (2011, background): “Similarly, Kenyon cells have been reported to generate a sparse and selective output in response to olfactory input (Perez-Orive et al., 2002; Wang et al., 2004; Szyszka et al., 2005; Jortner et al., 2007; Ito et al., 2008; Luo et al., 2010).”
Neuronal assemblies of the Drosophila mushroom body (2008, unknown): “4A of Wang et al., 2004).”
Event Timing in Associative Learning: From Biochemical Reaction Dynamics to Behavioural Observations (2012, methodology): “In the Drosophila brain, individual odours activate small, specific groups of Kenyon cells increasing their intracellular Ca concentration [22–24]; whereas shock induces a dopaminergic reinforcement signal, which is also delivered to the Kenyon cells [25–29].”
Imaging a Population Code for Odor Identity in the Drosophila Mushroom Body (2013, background): “Physiological recordings have shown that individual KCs exhibit highly odor-selective responses (Perez-Orive et al., 2002; Turner et al., 2008) and that odors induce sparse activation of the KC population (Wang et al., 2004; Honegger et al., 2011).”
Genetic dissection of neural circuits. (2008, unknown): “Recent development of recombineering technology (e.g., Warming et al., 2005) has made the construction of BACs nearly as convenient as conventional plasmids.”