Comprehensive Maps of Drosophila Higher Olfactory Centers: Spatially Segregated Fruit and Pheromone Representation

By Gregory S. X. E. Jefferis, Christopher Potter, Alexander M. Chan, E. Marin, Torsten Rohlfing, Calvin R. Maurer Jr., Liqun Luo. 2007

656 citations recorded by the source corpus.

Summary In Drosophila, ∼50 classes of olfactory receptor neurons (ORNs) send axons to 50 corresponding glomeruli in the antennal lobe. Uniglomerular projection neurons (PNs) relay olfactory information to the mushroom body (MB) and lateral horn (LH). Here, we combine single-cell labeling and image registration to create high-resolution, quantitative maps of the MB and LH for 35 input PN channels and several groups of LH neurons. We find (1) PN inputs to the MB are stereotyped as previously shown for the LH; (2) PN partners of ORNs from different sensillar groups are clustered in the LH; (3) fruit odors are represented mostly in the posterior-dorsal LH, whereas candidate pheromone-responsive PNs project to the anterior-ventral LH; (4) dendrites of single LH neurons each overlap with specific subsets of PN axons. Our results suggest that the LH is organized according to biological values of olfactory input.

Subfields: Cellular and Molecular Neuroscience, Genetics, Sensory Systems

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Citations in the corpus

Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila (2014, methodology): “In Drosophila, olfactory information is delivered to the MB by projection neurons from each of ∼50 antennal lobe glomeruli (Marin et al., 2002; Wong et al., 2002; Jefferis et al., 2007; Lin et al., 2007; Vosshall and Stocker, 2007; Yu et al., 2010).”
Recognition (2003, neutral): “Most PNs innervate a single glomerulus (Jefferis et al., 2007; Marin et al., 2002; Wong et al., 2002), while mitral/tufted cells project to the primary olfactory cortex (Price & Powell, 1970).”
The neuronal architecture of the mushroom body provides a logic for associative learning (2014, background): “Most antennal lobe projection neurons (PNs) extend dendrites to a single glomerulus and project axons that bifurcate to innervate two brain regions, the lateral horn and the MB (Stocker et al., 1990; Wong et al., 2002; Jefferis et al., 2007).”
Odor Discrimination in Drosophila: From Neural Population Codes to Behavior (2013, background): “, 2012), it remains unresolved whether innate odor responses in general reflect the activation of labeled lines that trigger hardwired behaviors (Gupta and Stopfer, 2012; Jefferis et al., 2007; Knaden et al., 2012; Semmelhack andWang, 2009).”
Different Kenyon Cell Populations Drive Learned Approach and Avoidance in Drosophila (2013, neutral): “The MB MP1 expressing c061:MBGAL80 is described in Krashes et al. (2009).”
Organization of antennal lobe‐associated neurons in adult Drosophila melanogaster brain (2012, background): “The medial antennal lobe tract (mALT) links the AL with the mushroom body (MB) calyx (CA) and lateral horn (LH), whereas the mediolateral antennal lobe tract (mlALT) connects the AL with the LH.”
Functional analysis of a higher olfactory center, the lateral horn (2012, background): “…and clustering in the projections of genetically labeled PNs in the LH (Tanaka et al., 2004; Jefferis et al., 2007; Lin et al., 2007; Kazama and Wilson, 2009), but whether dendrites of LHNs are restricted to these clusters remains controversial (Tanaka et al., 2004; Jefferis et al., 2007).”
The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing and Mosaic Analysis (2010, neutral): “Acj6, and acj6-GAL4, an enhancer trap line inserted into the acj6 locus, are also expressed in some GH146-negative anterodorsal PNs, in many olfactory receptor neurons (ORNs), in atypical PNs, and in lateral horn output neurons (Clyne et al., 1999; Komiyama et al., 2003; Suster et al., 2003; Komiyam”
Olfactory representations by Drosophila mushroom body neurons. (2008, background): “3H (Jefferis et al. 2007).”
Cellular-resolution population imaging reveals robust sparse coding in the Drosophila Mushroom Body (2011, background): “PNs send axons to distinct but rather large zones within the KC dendritic field in the calyx (Tanaka et al., 2004; Jefferis et al., 2007; Lin et al., 2007).”
A GAL4-driver line resource for Drosophila neurobiology. (2012, neutral): “…stochastic labeling, and mosaic analysis with a repressible cell marker analysis of GAL4 drivers, and screening of sparsely labeled enhancer trap GAL4 drivers (reviewed in Vosshall and Stocker, 2007; see, for example, Stocker et al., 1990; Jefferis et al., 2007; Tanaka et al., 2008, 2012).”
The SRI24 Multi-Channel Atlas of Normal Adult Human Brain Structure (2009, background): “More detailed atlases that focus on particular organs or anatomical regions are also common, such as the recently presented comprehensive map of the Drosophila olfactory system at the single-neuron level [Jefferis et al., 2007].”
Principles of connectivity among morphologically defined cell types in adult neocortex (2015, positive): “We extracted simple features from the axon and dendrite densitymaps of the reconstructed neurons using principal component analysis (11) and trained cross-validated sparse logistic regression classifiers to distinguish between any two types of interneurons as labeled manually (see the supplementary ”
Complementary Function and Integrated Wiring of the Evolutionarily Distinct Drosophila Olfactory Subsystems (2011, methodology): “For example, within the LH, PN partners of OSNs expressing fruit odor-responsive ORs have clustered axonal projections, and these segregate from PNs receiving input from candidate pheromone-responsive OSNs (Jefferis et al., 2007).”