Effect of partial substitution of dietary fish oil by vegetable oils on desaturation and β-oxidation of [1-14C]18:3n−3 (LNA) and [1-14C]20:5n−3 (EPA) in hepatocytes and enterocytes of European sea…
By G. Mourente, J. Dick, J. Bell, D. Tocher. 2005
140 citations recorded by the source corpus.
Abstract The increasing worldwide aquaculture output and concomitant decrease in the stocks of feed-grade fish used for fish oil production has made fish oil replacement in feeds a priority for the aquaculture industry. The regulation of fatty acid metabolism in fish is important in order to determine strategies for the best use of plant oils in diets for commercially important cultured fish species. We have studied the desaturation/elongation and β-oxidation of 14 C-linolenic (LNA) and 14 C-eicosapentaenoic (EPA) acids in hepatocytes and pyloric caecal enterocytes in European sea bass fed diets with partial substitution (60%) of fish oil (FO) with vegetable oils (rapeseed, linseed and palm oil) blended in different proportions, for 64 weeks. The rate of desaturation of 14 C-LNA was very low in hepatocytes from all treatments and no significant differences were observed among treatments. The rate of desaturation of 14 C-LNA in enterocytes was higher than that in hepatocytes but still low (less than 5% of total radioactivity recovered). The desaturation of 14 C-EPA in enterocytes was also higher than in hepatocytes, but again was low and no significant differences were found among treatments. The rates of β-oxidation of 14 C-LNA and 14 C-EPA were much higher than the rates of desaturation in both hepatocytes and enterocytes; however, no significant differences were observed in either hepatocytes or enterocytes among treatments. The rates of β-oxidation of 14 C-LNA were considerably higher than those of 14 C-EPA in both hepatocytes and enterocytes. In conclusion, European sea bass (a carnivorous marine fish) showed very low desaturation and elongation of LNA to EPA and DHA, and EPA to DHA, higher β-oxidation of LNA than EPA, and all desaturation and oxidation activities were significantly higher in enterocytes than in hepatocytes. A second major conclusion is that no clear quantitative nutritional effects on the desaturation/elongation and β-oxidation activities in either hepatocytes or enterocytes of sea bass were observed upon the inclusion of vegetable oils in the diet.
Subfields: Aquatic Science, Immunology, Physiology
View Paper: https://www.semanticscholar.org/paper/1c34f04518f63ffee85e94a792918ddf01c2ca62
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Citations in the corpus
- A comparative study on the effect of fish meal substitution with three different insect meals on growth, body composition and metabolism of European sea bass (Dicentrarchus labrax L.) (2020, unknown): “The lowering effect of EPA and DHA on cholesterol is well established in mammals (Connor, 2000; Phillipson et al., 1985).”
- Expression of fatty acyl desaturase and elongase genes, and evolution of DHA:EPA ratio during development of unfed larvae of Atlantic bluefin tuna (Thunnus thynnus L.) (2011, background): “Consistent with this, LC-PUFA synthesis in 450 hepatocytes or enterocytes from marine species including cod, and European and Asian sea bass, is 451 very low (Mourente et al., 2005; Tocher et al., 2006; Mohd-‐Yusof et al., 2010).”
- Characterization and modulation of gene expression and enzymatic activity of delta-6 desaturase in teleosts: A review (2011, background): “…have demonstrated that the inclusion of VOs did not lead to a significant increase in desaturase activity in sea bass hepatocytes (Mourente and Dick, 2002;Mourente et al., 2005b), while increased activity was reported in pyloric caecal enterocytes of sea bass fed VO blends (Mourente et al., 2005a).”