Large-scale phosphorylation analysis of mouse liver

By J. Villén, S. Beausoleil, S. Gerber, Steven P. Gygi. 2007

735 citations recorded by the source corpus.

Subfields: Materials Chemistry, Molecular Biology, Spectroscopy

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Citations in the corpus

Mammalian Ceramide Synthases (2010, background): “Data from high performance mass spectrometry support a suggestion that mouse liver CerS2 and CerS5 might be phosphorylated (33).”
AMPK phosphorylation of raptor mediates a metabolic checkpoint. (2008, background): “Notably, during the course of this study, two large-scale analyses of phosphoproteins from rat and mouse liver revealed phosphorylation of endogenous raptor at Ser722, suggesting that it is indeed a bona fide phosphorylation site in vivo (Moser and White, 2006; Villen et al., 2007).”
A tissue-specific atlas of mouse protein phosphorylation and expression. (2010, neutral): “Distribution of Phosphorylation Site Classes across Tissues and Phosphoproteins Phosphorylation sites were classified as acidic, basic, proline-directed, tyrosine, or other as described (Villen et al., 2007).”
Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. (2008, methodology): “The remaining part was directly digested prior to fractionation of the resulting peptides by strong cation exchange (SCX) chromatography (Beausoleil et al., 2004; Olsen et al., 2006; Villen et al., 2007).”
Decision Tree-Driven Tandem Mass Spectrometry for Shotgun Proteomics (2008, methodology): “Results Characterization of peptide identification probabilities To gather a training set of MS/MS spectra for probability calculations, we digested two aliquots of a whole-cell yeast lysate (that is, two biological replicates) using the protease endo-LysC and separated each into 12 fractio”
Phospho.ELM: a database of phosphorylation sites—update 2008 (2007, background): “For this reason, we consider it increasingly important to create and maintain publicly available phospho-protein databases, where the exponentially increasing number of known phosphorylation sites (7–12) can be easily accessed by the research community.”
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips (2007, methodology): “Enrichment of phosphopeptides in HeLa cytoplasmic extracts There have been only a few reports of the application of phosphopeptide enrichment methods to real, complex samples such as crude cell extracts including very recent large-scale studie”
The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry (2008, methodology): “However, the method that has proven most comprehensive in mapping the phosphoproteome is SCX chromatography combined with either IMA”
Proteomics by mass spectrometry: approaches, advances, and applications. (2009, methodology): “Proteome-scale screening of phosphotyrosines has been extended to the identification of oncogenic kinases (209), identification of core proteins responding to drug treatment (210), and definition of the organspecific phosphorylation (201).”