Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips
By Juri Rappsilber, Matthias Mann, Yasushi Ishihama. 2007
4,355 citations recorded by the source corpus.
Subfields: Molecular Biology, Spectroscopy
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- Matthias Mann
- Jüergen Cox
- Chunaram Choudhary
- Andriy Kryshtafovych
- Krzysztof Fidelis
- T. Geiger
- Christoph Schaab
- Juri Rappsilber
In Spectroscopy
- Matthias Mann
- Jüergen Cox
- Chunaram Choudhary
- Christoph Schaab
- Juri Rappsilber
- T. Geiger
- Andriy Kryshtafovych
- Nagarjuna Nagaraj
Citations in the corpus
- Small angle X-ray scattering and cross-linking for data assisted protein structure prediction in CASP 12 with prospects for improved accuracy (2018, methodology): “24 Resulting peptides were desalted using StageTips.”
- A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles (2011, methodology): “To further reduce the complexity of the immunoenriched samples, peptides were fractionated using isoelectric focusing (26), or a microcolumn-based strong cation exchange method (27).”
- Comparative Proteomic Analysis of Eleven Common Cell Lines Reveals Ubiquitous but Varying Expression of Most Proteins (2012, methodology): “Eluted peptides were concentrated and purified on C18 StageTips (20).”
- Dual Proteome-scale Networks Reveal Cell-specific Remodeling of the Human Interactome (2020, methodology): “5% HPLC-grade formic acid (Thermo Fisher Scientific) was subsequently added to quench digestion and samples were desalted with homemade stage tips as described previously (Rappsilber et al., 2007).”
- Assessment of chemical‐crosslink‐assisted protein structure modeling in CASP13 (2020, neutral): “Resulting peptides were desalted using StageTips.(13,14)”
- Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation. (2013, methodology): “2% trifluoroacetic acid [TFA]) and loaded directly onto a strong cation exchange (SCX) microtip column prepared as described (Rappsilber et al., 2007; Wi sniewski et al., 2009).”
- Deep proteome and transcriptome mapping of a human cancer cell line (2011, methodology): “The peptides were purified on StageTips (Rappsilber et al, 2007).”