Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.

By H. Daub, Jesper V. Olsen, Michaela Bairlein, Florian Gnad, Felix S. Oppermann, R. Körner, Z. Greff, G. Kéri, O. Stemmann, Matthias Mann. Molecules and Cells, 2008

618 citations recorded by the source corpus.

Subfields: Cell Biology, Materials Chemistry, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/87d6be5111267c635b52694ccd62ed611a23d054

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Citations in the corpus

Quantitative Phosphoproteomics Reveals Widespread Full Phosphorylation Site Occupancy During Mitosis (2010, methodology): “S1B); 78% overlap with our previous time-resolved EGF phosphoproteome study (11) and 51% overlap with a study of phosphorylation sites on samples enriched for HeLa kinases (15).”
Proteomics by mass spectrometry: approaches, advances, and applications. (2009, methodology): “Recent studies quantified both phosphorylation levels and protein levels for mitotic phosphorylation (223), cell cycle kinases (251), and the beta-adrenergic pathway (206) (Figure 6).”
Decoding signalling networks by mass spectrometry-based proteomics (2010, methodology): “To obtain an in-depth coverage of kinase phosphorylation, a chemical proteomics approach using immobilized ATP mimetics was used to enrich protein kinases, which allowed SILAC quantification of their phosphorylation sites in mitosi”
Comprehensive molecular portraits of human breast tumors (2012, neutral): “Mutations in RUNX1 and its dimerization partner CBFB may also have a role in aberrant ER signalling in luminal tumours, as RUNX1 functions as an ER ‘DNA tethering factor’ 32 .”
Dynamic Reprogramming of the Kinome In Response to Targeted MEK Inhibition In Triple Negative Breast Cancer (2012, positive): “Profiling kinase activity in tumors and cell lines was carried out using Multiplexed Inhibitor Beads (MIBs), which consist of mixtures of Sepharose beads with covalently immobilized, linker adapted, kinase inhibitors of moderate selectivity for different kinases (Bisindoylmaleimide-X, SB203580, Dasa”
The MAPK cascades: signaling components, nuclear roles and mechanisms of nuclear translocation. (2011, background): “Currently, the number of identi ﬁ ed substrates is as high as 200 (see initial list in [5]), although recent studies indicate that the actual number is likely to be much higher [83].”
PHOSIDA 2011: the posttranslational modification database (2010, neutral): “The current version of PHOSIDA contains an additional 20 000 human phosphorylation sites from kinase enriched samples (21) or of quantified dynamics during the cell cycle (5,7).”