The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes

By Ji‐Joon Song, Jidong Liu, Niraj Tolia, J. Schneiderman, Stephanie K. Smith, R. Martienssen, Gregory J. Hannon, L. Joshua-Tor. Nature Structural Biology, 2003

610 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/167c3612bf966ea579b9311bf19aafa063b4ee11

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In Molecular Biology

Citations in the corpus

Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. (2006, background): “…domain of Dicer may interact with the 30 overhang at the terminus and determines the processing site in a ruler-like fashion that measures 22 nt segments away from the terminus (Lingel et al., 2004; Ma et al., 2004; Macrae et al., 2006; Song et al., 2003; Vermeulen et al., 2005; Yan et al., 2003).”
Second-generation shRNA libraries covering the mouse and human genomes (2005, background): “This probably involves recognition of the 2-nt 3′ overhang created by Drosha to focus Dicer cleavage at a single site ∼22 nt from the end of the hairpi”
Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha (2005, background): “The two RNaseIII domains in Drosha are similar in size, and Drosha does not have the PAZ protein domain that mediates Dicer recognition of the base of the pre-miRNA stem (Lingel et al, 2003; Song et al, 2003; Yan et al, 2003).”
MicroRNA: Biogenesis, Function and Role in Cancer (2010, background): “The PAZ domain, RNase III nuclease domains and the dsRBD are involved in the binding and cleavage of dsRNA [107, 113-116].”
Control of translation and mRNA degradation by miRNAs and siRNAs. (2006, background): “Argonaute proteins directly interact with the miRNA/siRNA (Song et al. 2003; Ma et al. 2004, 2005).”
The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. (2004, background): “ARGONAUTE proteins (also known as PPD proteins because they carry PAZ and PIWI domains) have a -barrel core that together with a side appendage weakly bind to single-stranded RNAs at least 5 nt in length and also to double-stranded RNA (Lingel et al. 2003; Song et al. 2003; Yan et al. 2003), suggest”
RNA meets chromatin. (2005, background): “The RNAi machinery contains RNA-binding proteins that guide and transport RNA, such as the Argonaute family of proteins that bind small RNAs through their PAZ (piwi/argonaute/zwille) domain (Lingel et al. 2003; Song et al. 2003; Yan et al. 2003; Ma et al. 2004).”
Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain (2004, background): “The PAZ domain is an RNA-binding module found in Argonaute and some Dicer proteins and its structure has been determined in the free stat”
Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing. (2006, background): “To gain insight into how 29-Omethyl modification at position 2 affects off-target silencing, we examined published structures of siRNA guide strands bound to components of RISC (Song et al. 2003; Ma et al. 2005; Parker et al. 2005).”
microPrimer: the biogenesis and function of microRNA (2005, background): “Structural studies show that the PIWI domain binds to small RNAs at their 5 end, whereas the PAZ domain binds to the 3 end of single-stranded RNAs (Song et al., 2003; Yan et al., 2003; Lingel et al., 2004; Parker et al., 2004; Ma et al., 2005; Parker et al., 2005).”