The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development.

By H. Vaucheret, Franck Vazquez, P. Crété, David P. Bartel. Genes & Development, 2004

1,016 citations recorded by the source corpus.

MicroRNAs (miRNAs) are endogenous 21-24-nt RNAs that can down-regulate gene expression by pairing to the messages of protein-coding genes to specify mRNA cleavage or repression of productive translation. They act within the RNA-induced silencing complex (RISC), which in animals contains a member of the Argonaute family of proteins. In the present study, we show that Arabidopsis ago1 mutants have increased accumulation of mRNAs known to be targeted for cleavage by miRNAs. In hypomorphic ago1 alleles, this compromised miRNA function occurs without a substantial change in miRNA accumulation, whereas in null alleles it is accompanied by a drop in some of the miRNAs. Therefore, AGO1 acts within the Arabidopsis miRNA pathway, probably within the miRNA-programmed RISC, such that the absence of AGO1 destabilizes some of the miRNAs. We also show that targeting of AGO1 mRNA by miR168 is needed for proper plant development, illustrating the importance of feedback control by this miRNA. Transgenic plants expressing a mutant AGO1 mRNA with decreased complementarity to miR168 overaccumulate AGO1 mRNA and exhibit developmental defects partially overlapping with those of dcl1, hen1, and hyl1 mutants showing a decrease in miRNA accumulation. miRNA targets overaccumulate in miR168-resistant plants, suggesting that a large excess of AGO1 protein interferes with the function of RISC or sequesters miRNAs or other RISC components. Developmental defects induced by a miR168-resistant AGO1 mRNA can be rescued by a compensatory miRNA that is complementary to the mutant AGO1 mRNA, proving the regulatory relationship between miR168 and its target and opening the way for engineering artificial miRNAs in plants.

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Citations in the corpus

Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. (2008, background): “Many processes, such as leaf development, auxin signaling, phase transition, flowering, and genome maintenance, are regulated in similar ways by different miRNAs (Aukerman and Sakai 2003; Palatnik et al. 2003; Vaucheret et al. 2004; Guo et al. 2005; Mallory et al. 2005).”
Regulation of AUXIN RESPONSE FACTOR3 by TAS3 ta-siRNA affects developmental timing and patterning in Arabidopsis. (2006, background): “With the assumption that AGO7 is similar to AGO1 [11] and AGO4 [12, 13], small RNAs that function in association with AGO7 are generally predicted to be destabilized and accumulate to low or nondetectable levels in zip-1 mutant plants.”
Plant microRNA: a small regulatory molecule with big impact. (2006, background): “Increased target mRNA suggested that non-functional RISCs were formed in the mutant plants (Vaucheret et al., 2004).”
Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3 (2006, background): “…of SPL3 increases with time, transgenes undergoing silencing are either permanently silenced very early in shoot development, or display progressively lower levels of expression during shoot growth (de Carvalho et al., 1992; Glazov et al., 2003; Palauqui et al., 1996; Vaucheret et al., 2004).”
Plant microRNAs: new players in functional genomics (2013, unknown): “Among the 10 AGO family members, AGO1 was found to be one of the most important members for miRNA biogenesis and processing in plants (Vaucheret et al. 2004; Kinder and Martienssen 2005; Ronemus et al. 2006; Mi et al. 2008; Takeda et al. 2008).”
Small RNAs and Their Roles in Plant Development (2009, background): “AGO1, which encodes the major effector protein in the miRISC, is itself under the control of miR168 (Vaucheret et al. 2004).”
microPrimer: the biogenesis and function of microRNA (2005, background): “DCL1 and AGO1 themselves are also miRNA targets, suggesting a negative-feedback mechanism in which miRNAs tune their own expression (Rhoades et al., 2002a; Xie et al., 2003; Vaucheret et al., 2004).”
MicroRNAS and their regulatory roles in plants. (2006, background): “miR168 ARGONAUTE (116) 1 6 2 AGO1 (131, 133) 5′ RACE, miRNA-resistant”
A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. (2006, methodology): “RNA gel blot analysis of small RNA expression RNA gel blots for miRNAs were performed using mutants and protocols as described previously (Vaucheret et al. 2004; Vazquez et al. 2004).”
Gardening the genome: DNA methylation in Arabidopsis thaliana (2005, background): “A further complication is that dicer-like 1 ( dcl1 ) and argonaute 1 ( ago1 ) mutants have severe developmental defects because the encoded proteins are important for microRNA-mediated regulation of embryonic developmen”
Functions of microRNAs and related small RNAs in plants (2006, background): “thaliana , where the mature single-stranded miRNA guides the RNA slicing activity of AGO1 to partially complementary mRNA”