Second-generation shRNA libraries covering the mouse and human genomes

By J. M. Silva, Mamie Z. Li, Ken Chang, W. Ge, Michael C. Golding, Richard J. Rickles, D. Siolas, G. Hu, Patrick J. Paddison, M. Schlabach and 10 more. Nature Genetics, 2005

678 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a16562f7ef79826da95508016aea1e00ae29c4f3

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Citations in the corpus

WNT/TCF signaling through LEF1 and HOXB9 mediates lung adenocarcinoma metastasis (2009, methodology): “These were cloned into a modified pSM2 mir based vector, which expresses the short hairpin embedded in a larger microRNA sequence (Silva et al., 2005).”
The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo (2011, background): “We have further generated genomewide shRNA libraries in these vectors that cover large numbers of mammalian transcripts for systematic study of gene function (5, 6).”
A viral microRNA functions as an orthologue of cellular miR-155 (2007, background): “This arrangement of the miR-30 flanking regions was originally described by Silva et al.(27) and results in unique XhoI and”
Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. (2006, background): “Recent breakthroughs in RNAi technology have been made by generating shRNA expression cassettes that can mimic a natural miRNA gene (Dickins et al., 2005; Silva et al., 2005; Zeng et al., 2002).”
Essential metabolic, anti-inflammatory, and anti-tumorigenic functions of miR-122 in liver. (2012, positive): “As negative control, a vector lacking a miRNA (scAAV.eGFP) or a vector expressing a synthetic miRNA targeting firefly luciferase expressed from a miR-30 backbone as previously described (36) (scAAV.shLuc.eGFP) was ics of hepatocyte differentiation in a manner that is no longer evident at birth.”
Interferon Receptor Signaling Pathways Regulating PD-L1 and PD-L2 Expression (2017, positive): “Plasmids were prepared using the PureLink HiPure Filter Plasmid Maxiprep Kit (Invitrogen) or NucleoSpin 96-well Miniprep kits (Macherey-Nagel), and lentiviral particles were produced as previously described (Kappes and Wu, 2001; Silva et al., 2005).”
A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells (2009, background): “The pENTR/pSM2 plasmids are based on the pSM2c plasmid [14,25] for expression of miRNAs, and include the 59 and 39 flanking sequences from the miR-30 miRNA.”
A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. (2006, methodology): “Another group screened by transfecting cells with shRNA-expressing retroviral plasmids and identified genes involved in proteasome function (Paddison et al., 2004; Silva et al., 2005).”
Strategies for silencing human disease using RNA interference (2007, background): “Although the effector stages of this endogenous pathway have not been used for therapeutic development, perfect duplex siRNA sequences have been introduced into pri-miRNA and pre-miRNA backbones, generating miRNA mimics that are processed by the miRNA pathway but trigger the more potent PTGS pathway”
Gene silencing in plants using artificial microRNAs and other small RNAs. (2008, methodology): “Among the most successful variants are short-hairpin RNAs (shRNAs), which are based on miRNA precursor backbones, the processing of which is understood in great detail (Chang et al., 2006; Paddison et al., 2002; Silva et al., 2005).”
Transcripts Targeted by the MicroRNA-16 Family Cooperatively Regulate Cell Cycle Progression (2007, background): “expressed as a synthetic primary miRNA-like hairpin (46).”