A custom microarray platform for analysis of microRNA gene expression

By J. Thomson, J. Parker, Charles M. Perou, Scott M. Hammond. Nature Methods, 2004

749 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/1324947406056796b5a2404f91a694c4686defb4

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Citations in the corpus

An overview of microRNAs (2015, methodology): “Early studies were performed using microarray, RT-PCR, and bead-based hybridization (Luminex) platforms, while more recent studies have used NGS-based profiling[117, 162-167].”
Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. (2008, methodology): “Target RNA labeling was performed as described (Thomson et al. 2004).”
The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation (2006, methodology): “To understand the potential involvement of miRNAs in skeletal muscle proliferation and differentiation, we analyzed the expression of miRNAs during skeletal muscle differentiation using an established microarray analysi”
A novel and universal method for microRNA RT-qPCR data normalization (2009, background): “Despite the small size of miRNA molecules, several technologies have been developed that enable high-throughput and sensitive miRNA profiling, such as microarrays [3-8], real-time quantitative PCR (RT-qPCR) [9,10] and bead-based flow cytometry [2].”
microRNAs as oncogenes and tumor suppressors. (2007, methodology): “The highest levels of let-7 expression occur in differentiated adult tissues (Miska et al., 2004; Thomson et al., 2004).”
Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis. (2007, methodology): “10 μg of total RNA isolated using Trizol (Invitrogen) was labeled with Cy3 as described (Thomson et al., 2004) and hybridized to arrays at 37° overnight in 400mM Na2HPO4 (pH 7.”
Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. (2005, methodology): “Other systems have recently been described for the expression profiling of miRNAs (Babak et al. 2004; Liu et al. 2004; Miska et al. 2004; Nelson et al. 2004; Thomson et al. 2004).”
MiRNA-Directed Regulation of VEGF and Other Angiogenic Factors under Hypoxia (2006, methodology): “Fluorescein-labeled miRNA [36] were used for hybridization on each miRNA microarray chip containing 509 probes in triplicate, corresponding to 435 human (including 122 predicted miRNAs), 261 mouse, and 196 rat miRNAs found in the miRNA Registry (http://microrna.”
Genomics of microRNA. (2006, background): “Cy3 and Cy5 Mature miRNA CC Human 26 tissues [94] Cy3 and Cy5 Mature miRNA CC Mouse Four developmental stages, seven tissues, ES cell [ 93 ]”
Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing. (2008, methodology): “MicroRNA expression levels were examined by quantitative realtime PCR (qRT-PCR) as well as by microRNA microarray as previously reported (Thomson et al. 2004, 2006).”