Dynamic binding of histone H1 to chromatin in living cells

By Tom Misteli, A. Gunjan, R. Hock, M. Bustin, David T Brown. Nature, 2000

652 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/fb47538b1d2fc62ba6a71540ed15a2b8b9422119

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In Molecular Biology

Citations in the corpus

Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms (2020, result): “These results are consistent with the observations from the fluorescence recovery after photobleaching (FRAP) experiments, which showed that linker histones have much shorter residence time than the core histones (Misteli et al., 2000), and the local resolution of the H1 globular domain is lower tha”
The Major Architects of Chromatin: Architectural Proteins in Bacteria, Archaea and Eukaryotes (2008, background): “The globular domain and C-terminal tail of H1 are required for high affinity binding to chromatin and H1 was shown to interact with chromatin transiently in the order of minutes (Lever et al., 2000; Misteli et al., 2000).”
The genomic landscape of the somatic linker histone subtypes H1.1 to H1.5 in human cells. (2013, methodology): “Third, DamID is a suitable method for proteins with relatively short residence times (Gelbart et al., 2005; Schmiedeberg et al., 2009; Misteli et al., 2000) because the adenine methylation remains after”
Release of chromatin protein HMGB1 by necrotic cells triggers inflammation (2002, background): “By contrast, in HeLa cells that expressed GFP fusions of chromatin proteins HMGN1 and HMGN2, or of transcription factor NF1, fluorescence loss was significantly slower, and in cells expressing GFP–histone H1c fusions fluorescence loss was very limited (ref”
The nucleosome: from genomic organization to genomic regulation. (2004, neutral): “In the fol-tion from nucleosomes (Misteli et al., 2000).”
Revisit of reconstituted 30-nm nucleosome arrays reveals an ensemble of dynamic structures (2018, background): “For example, FRAP experiments revealed that the residence time of linker histones in chromatin is short, on the order of a minute [44], suggesting that chromatin containing linker histones has a dynamic structure.”
γ-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin (2008, background): “The further condensation of the 30 nm fiber as well as higher levels of chromatin condensation, which culminate with the 10 000-fold compaction of the stretched DNA fiber in the 700 nm metaphase chromosomes, are less well understood (5), but are facilitated by the linker histone H1 and condensins (6”
A dual role of linker histone H1.4 Lys 34 acetylation in transcriptional activation. (2012, background): “However, in vivo H1 has been shown to be more dynamic than core histones (Lever et al. 2000; Misteli et al. 2000).”
Global transcription in pluripotent embryonic stem cells. (2008, neutral): “IF was performed as described (Misteli et al., 2000).”
Kinetics of Core Histones in Living Human Cells (2001, methodology): “This approach has been used with H2A, H2B, and the linker histone H1 (Kanda et al., 1998; Dey et al., 2000, Lever et al., 2000, Misteli et al., 2000; Perche et al., 2000; Phair and Misteli, 2000).”
Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells. (2006, background): “IF was performed as described (Misteli et al., 2000).”
How do site-specific DNA-binding proteins find their targets? (2004, background): “For example, FRAP ( ̄uorescence recovery after photobleaching) experiments have shown that, in living cells, histone H1 transfers between its DNA-binding sites via free solution (66,67); likewise, for a repair endonuclease between sites of DNA damage (65).”