Cd11c+B220+Gr-1+ Cells in Mouse Lymph Nodes and Spleen Display Characteristics of Plasmacytoid Dendritic Cells

By H. Nakano, M. Yanagita, M. Gunn. Journal of Experimental Medicine, 2001

815 citations recorded by the source corpus.

Human plasmacytoid dendritic cells (pDCs) are major producers of IFNα, are activated by CpG motifs, and are believed to enter lymph nodes (LNs) via L-selectin dependent extravasation across high endothelial venules. To identify a similar murine DC type, CD11c+ cells in the LNs of L-selectin–deficient and control BALB/c mice were compared, revealing a population of CD11c+CD11b− cells that is reduced 85% in the LNs of L-selectin–deficient mice. These cells are Gr-1+B220+CD19−, either CD4+ or CD8+, and localize within T cell zones of LNs. Freshly isolated CD11c+Gr-1+ cells express major histocompatibility complex class II at low levels, display a plasmacytoid morphology, and survive poorly in culture. Their survival is increased and they develop a DC-like morphology in interleukin 3 and CpG. Like human pDCs, CD11c+Gr-1+ cells stimulate T cell proliferation after activation with CpG and produce IFNα after stimulation with influenza virus. These cells also display a strain-specific variation in frequency, being fivefold increased in the LNs of BALB/c relative to C57BL/6 mice. These CD11c+CD11b−B220+Gr-1+ cells appear to be the murine equivalent of human pDCs.

Subfields: Immunology, Immunology and Allergy

View Paper: https://www.semanticscholar.org/paper/fa00828ce64915520c0eaf51dc92c69ed950531a

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Citations in the corpus

Toll-like receptors. (2003, background): “These findings also indicate 348 TAKEDA ¥ KAISHO ¥ AKIRA that TLR9 directly recognizes CpG DNA.”
Plasmacytoid dendritic cells in immunity (2004, background): “Characterization of mouse pDCs In 2001, three different groups reported the identification of mouse pDC”
IRFs: master regulators of signalling by Toll-like receptors and cytosolic pattern-recognition receptors (2006, background): “Recently, much attention has been focused on the large amounts of type I IFNs that are produced by plasmacytoid DCs (pDCs”
Viral infection switches non-plasmacytoid dendritic cells into high interferon producers (2003, background): “For example, influenza, widely used to stimulate production of IFN-I by PDC”
The Development of Murine Plasmacytoid Dendritic Cell Precursors Is Differentially Regulated by FLT3-ligand and Granulocyte/Macrophage Colony-Stimulating Factor (2002, background): “Because mouse IPCs were recently shown to express CD11c, B220, GR-1, CD45RB, and Ly6c, but not CD11b (3, 4, 5), we investigated whether the CD11c cells generated in culture contained cells with IPC phenotype by three color flow”
Mouse and human dendritic cell subtypes (2002, background): “A second mouse DC precursor has recently been isolated from mouse lymphoid tissue”
Cross‐presentation, dendritic cell subsets, and the generation of immunity to cellular antigens (2004, background): “like receptor (TLR) ligands, such as CpG, or through detection of virions (90, 91, 97).”