Quantification of biofilm structures by the novel computer program COMSTAT.

By A. Heydorn, A. T. Nielsen, M. Hentzer, C. Sternberg, M. Givskov, Bjarne Kjær Ersbøll, Søren Molin. Microbiology, 2000

2,185 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Periodontics

View Paper: https://www.semanticscholar.org/paper/eff3d240b96d9409437a687c7e7f7797a23cd0c0

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. M. Parsek
2. L. Hall-Stoodley
3. Søren Molin
4. M. E. Davey
5. P. Stoodley
6. M. Givskov
7. G. O’Toole
8. George - John Nychas

In Molecular Biology

1. M. Givskov
2. T. Bjarnsholt
3. M. Parsek
4. Søren Molin
5. P. Stoodley
6. L. Hall-Stoodley
7. P. Watnick
8. E. Karatan

In Periodontics

1. M. Parsek
2. L. Hall-Stoodley
3. M. Simões
4. P. Watnick
5. P. Stoodley
6. M. Givskov
7. M. Hentzer
8. E. Karatan

Citations in the corpus

• Biofilms: At the Interface between Biophysics and Microbiology (2006, methodology): “dimension, average and maximum diffusion distance, and surface-to-volume ratio [147].”
• Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system. (2003, background): “…Kolter, 1998a), the flagellummediated recruitment of planktonic cells by the developing biofilm from the liquid medium (Tolker-Nielsen et al., 2000) or the formation and growth of microcolonies formed as a consequence of the multiplication of cells attached to solid surfaces (Heydorn et al., 2000).”
• Bacterial biofilms: from the Natural environment to infectious diseases (2004, methodology): “Quantification is being addressed with the development of image analysis packages, such as COMSTA”
• Heavy Metal Resistance of Biofilm and Planktonic Pseudomonas aeruginosa (2003, methodology): “ysis program COMSTAT (14) was modified to include a subroutine capable of”
• Autoinducer 2 Controls Biofilm Formation in Escherichia coli through a Novel Motility Quorum-Sensing Regulator (MqsR, B3022) (2006, methodology): “determined with COMSTAT image-processing software (22) written as a script”
• Online Biofilm Monitoring (2003, methodology): “Nivens et al. already pointed out the potential of this method for industrial application in 1995, and work on this field continues (Heydorn et al. 2000; Xavier et al. 2000, 2003).”
• Statistical Analysis of Pseudomonas aeruginosa Biofilm Development: Impact of Mutations in Genes Involved in Twitching Motility, Cell-to-Cell Signaling, and Stationary-Phase Sigma Factor Expression (2002, methodology): “aeruginosa strains in the present study, we used a recently developed computer program, COMSTAT (12).”
• Rhamnolipid Surfactant Production Affects Biofilm Architecture in Pseudomonas aeruginosa PAO1 (2003, methodology): “the COMSTAT image analysis software package (11, 12).”
• Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms (2009, background): “aeruginosa PAO1 makes flat and compact biofilms in flow chambers when grown on citrate, benzoate, and Casamino Acids as carbon sources, whereas it makes irregular biofilms with the typical mushroom-shaped structures when glucose is used as a carbon source (132, 175, 304).”
• Chemical and physical methods for characterisation of biofilms (2007, neutral): “structures are presented in the literature [23–25].”
• Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function (2001, methodology): “lars of cells separated by spaces devoid of bacteria (5, 21).”
• Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants (2003, methodology): “Competition experiments, in which biofilms were initiated with 1:1 mixtures of Yfptagged DfliM mutant and Cfp-tagged wild type (and the opposite colour combination), and the biomass accumulation of each strain in the developing biofilm was quantified by COMSTAT analysis, showed that the DfliM mutant”