Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement

By V. Westphal, S. Rizzoli, M. Lauterbach, D. Kamin, R. Jahn, Stefan W. Hell. Science, 2008

805 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Biophysics

View Paper: https://www.semanticscholar.org/paper/e80d4ecdeaa8cc0ca713d97a5e87d590edb5dccf

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Citations in the corpus

Diamond-based single-photon emitters (2011, neutral): “vesicles in living neurons [196].”
A guide to super-resolution fluorescence microscopy (2010, methodology): “…al., 2007; Bock et al., 2007; Shroff et al., 2007; Bossi et al., 2008; Gunkel et al., 2009; Subach et al., 2009; van de Linde et al., 2009) and has been successfully used to map vertebrate kinetochore components Nägerl et al., 2008; Westphal et al., 2008; Eggeling et al., 2009; Opazo et al., 2010).”
STED microscopy reveals crystal colour centres with nanometric resolution. (2009, background): “, and for the real-time imaging of colloidal particles or synaptic vesicles in living neuron”
Super resolution fluorescence microscopy (2009, methodology): “More recently, they have demonstrated video-rate (28 fps) imaging of live hippocampal neurons and observed the movement of individual synaptic vesicles with 60–80nm resolution (64).”
Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics (2015, methodology): “High resolutionwith STED or RESOLFT demands high factors of saturated depletion (fig.”
Fast three-dimensional super-resolution imaging of live cells (2011, background): “Recent demonstrations of super-resolution imaging in living cells, as exemplified by the video-rate STED imaging of synaptic vesicles in live neuron”
Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A–C (2010, methodology): “The velocities of the vesicles were automatically analyzed using a previously described algorithm with MATLAB (The MathWorks, Inc.; Westphal et al., 2008).”
Microscopy and its focal switch (2008, background): “, as has been demonstrated by imaging synaptic vesicles in living neurons at video rat”
Single-cell biological lasers (2011, methodology): “Stimulated emission is an emerging scheme to improve the resolution and sensitivity of microscopic imaging in biomedical scienc”
Super-resolution Imaging of Chemical Synapses in the Brain (2010, positive): “Impressive video-rate imaging has been achieved with STED, making it a particularly powerful approach for probing fast dynamics in small brain structures, such as a dendritic spine (Nägerl et al., 2008; Westphal et al., 2008).”
Far-Field Optical Nanoscopy (2007, background): “Recent fast galvanometer-scanning implementations of STED microscopy [93] visualized the rapid motion of dense synaptic vesicles at the synapse of living hippocampal neurons at video rate [94].”
Lattice models of nonequilibrium bacterial dynamics (2010, background): “For instance, recent methods of nonlinear optics have made possible observation of biological systems on much smaller scales than was previously possible [1].”