A guide to super-resolution fluorescence microscopy

By Lothar Schermelleh, Rainer Heintzmann, H. Leonhardt. Journal of Cell Biology, 2010

1,272 citations recorded by the source corpus.

For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.

Subfields: Biomedical Engineering, Biophysics

View Paper: https://www.semanticscholar.org/paper/e7ffef3483f3e8e13ecc71d69b4e4a6b88d3847a

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In Biomedical Engineering

1. E. Rogers
2. Junhong Min
3. M. Gerl
4. Nikolay I. Zheludev
5. Suliana Manley
6. Michael A. Unser
7. K. Simons

In Biophysics

1. E. Rogers
2. M. Gerl
3. Nikolay I. Zheludev
4. Suliana Manley
5. Michael A. Unser
6. K. Simons

Citations in the corpus

• More than just a focus: The chromatin response to DNA damage and its role in genome integrity maintenance (2011, background): “Thus, the DDR field needs to dissect the 'focus' at the higher analytical and dynamic level; the emerging 'super-resolution' technologies are promisin”
• Optical super-oscillations: sub-wavelength light focusing and super-resolution imaging (2013, background): “In contrast, the promising, and commercially available, technique of stimulated emission depletion microscopy [81–83], is more optically complex, in that it requires two beams of different wavelength to be aligned with each other in three dimensions and, while it can operate at high speed, it requir”
• Revitalizing membrane rafts: new tools and insights (2010, methodology): “Moreover, super-resolution optical microscopy methods have been introduced that provide resolution well beyond the diffraction limi”
• Quantitative evaluation of software packages for single-molecule localization microscopy (2015, background): “At the cost of a relatively simple setup 6,7 , it has opened exciting new opportunities in life science research 8,9 .”