Super resolution fluorescence microscopy
By Bo Huang, M. Bates, X. Zhuang. Annual Review of Biochemistry, 2009
1,342 citations recorded by the source corpus.
Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.
Subfields: Biomedical Engineering, Biophysics
View Paper: https://www.semanticscholar.org/paper/53341cfa5ed031a47444514f50c5436c1993183a
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In Biomedical Engineering
Citations in the corpus
- Super Resolution Laser Radar with Blinking Atmospheric Particles - Application to Interacting Flying Insects (2014, neutral): “While the STED approach is based on a complicated laser scheme, other approaches, photoactivation localization microscopy (PALM) [28] and stochastic optical reconstruction microscopy (STORM) [29, 30], are based on sensitive cameras, single molecule detection and blinking molecules.”
- Deep learning in bioinformatics: introduction, application, and perspective in big data era (2019, background): “In biology, due to the limitation of the imaging acquiring technology, the obtained images are often noisy and of low resolution, such as Cryo-EM images [102] and fluorescence images [59], which requires postprocessing to obtain high-resolution, noise-free images.”