Super-resolution Imaging of Chemical Synapses in the Brain
By Adish Dani, Bo Huang, J. Bergan, C. Dulac, X. Zhuang. Neuron, 2010
701 citations recorded by the source corpus.
Determination of the molecular architecture of synapses requires nanoscopic image resolution and specific molecular recognition, a task that has so far defied many conventional imaging approaches. Here we present a super-resolution fluorescence imaging method to visualize the molecular architecture of synapses in the brain. Using multicolor, three-dimensional stochastic optical reconstruction microscopy, the distributions of synaptic proteins can be measured with nanometer precision. Furthermore, the wide-field, volumetric imaging method enables high-throughput, quantitative analysis of a large number of synapses from different brain regions. To demonstrate the capabilities of this approach, we have determined the organization of ten protein components of the presynaptic active zone and the postsynaptic density. Variations in synapse morphology, neurotransmitter receptor composition, and receptor distribution were observed both among synapses and across different brain regions. Combination with optogenetics further allowed molecular events associated with synaptic plasticity to be resolved at the single-synapse level.
Subfields: Atomic and Molecular Physics, and Optics, Neurology, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/0c59d8fb198cf5414eb13a0d43c0f657fb40f65a
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In Atomic and Molecular Physics, and Optics
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Citations in the corpus
- Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging (2011, positive): “…pairs may be implemented using the highest performing dye (Alexa 647, or its close structural analogs), which performs far better than fluorophores in the other spectral channels, thus enabling multi-color super-resolution imaging with high resolution in all color channels 6,12,41 .”
- Mechanisms of CaMKII action in long-term potentiation (2012, background): “Synaptic PSD95 has been visualized by electron microscope tomography and is oriented perpendicular to the membrane with the PDZ1 and PDZ2 domains that bind stargazin situated quite far (15 nm) from the membran”