Microscopy and its focal switch

By Stefan W. Hell. Nature Methods, 2008

977 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Biophysics, Electrical and Electronic Engineering

View Paper: https://www.semanticscholar.org/paper/33fdf2e01853e051985115c6f4435f8575607637

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Citations in the corpus

Tracking in cell and developmental biology. (2009, background): “On the hardware side, advances in optical systems design have taken light microscopy from widefield to (multiphoton) confocal and spinning disk microscopy [5] [6], and more recent efforts to break the diffraction barrier have further extended the palette [7] [8].”
Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective (2012, background): “, switched ON (fluorescent) and OFF (non-fluorescent) with appropriately selected laser beams in a sequential manner [2129].”
Direct stochastic optical reconstruction microscopy with standard fluorescent probes (2011, background): “Even though all concepts are very similar and excellent reviews have already been published highlighting the strengths and limitations of the different method”
Brief update on endocytosis of nanomedicines (2019, background): “It is now possible to look deep inside cells past the previously restrictive diffraction limit of optical microscopes and into the realm of super resolution fluorescent microscopy [218,219].”
Image deblurring with Poisson data: from cells to galaxies (2009, methodology): “In astronomy, it is a charged coupled device (CCD) camera while, in fluorescence microscopy, it can be also a photomultiplier tube (PMT) or an avalanche photo-diode (APD), as in the case of confocal, 4Pi, two-photon excitation and other microscopy techniques [48, 67].”
Spatially resolved, highly multiplexed RNA profiling in single cells (2015, positive): “Last, theoretical predictions (17) indicate that the use of superresolution imaging (41, 42) could increase the resolvable density to ~10(5) molecules/mm(3), which should be ample to address the entire transcriptome, even in cell types with RNA densities substantially higher than that of IMR90.”