Gut Microbiota in Human Adults with Type 2 Diabetes Differs from Non-Diabetic Adults

By N. Larsen, F. Vogensen, Frans W. J. van den Berg, D. Nielsen, A. S. Andreasen, B. K. Pedersen, W. Al-Soud, S. Sørensen, L. Hansen, M. Jakobsen. PLoS ONE, 2010

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Background Recent evidence suggests that there is a link between metabolic diseases and bacterial populations in the gut. The aim of this study was to assess the differences between the composition of the intestinal microbiota in humans with type 2 diabetes and non-diabetic persons as control. Methods and Findings The study included 36 male adults with a broad range of age and body-mass indices (BMIs), among which 18 subjects were diagnosed with diabetes type 2. The fecal bacterial composition was investigated by real-time quantitative PCR (qPCR) and in a subgroup of subjects (N = 20) by tag-encoded amplicon pyrosequencing of the V4 region of the 16S rRNA gene. The proportions of phylum Firmicutes and class Clostridia were significantly reduced in the diabetic group compared to the control group (P = 0.03). Furthermore, the ratios of Bacteroidetes to Firmicutes as well as the ratios of Bacteroides-Prevotella group to C. coccoides-E. rectale group correlated positively and significantly with plasma glucose concentration (P = 0.04) but not with BMIs. Similarly, class Betaproteobacteria was highly enriched in diabetic compared to non-diabetic persons (P = 0.02) and positively correlated with plasma glucose (P = 0.04). Conclusions The results of this study indicate that type 2 diabetes in humans is associated with compositional changes in intestinal microbiota. The level of glucose tolerance should be considered when linking microbiota with metabolic diseases such as obesity and developing strategies to control metabolic diseases by modifying the gut microbiota.

Subfields: Food Science, Infectious Diseases, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e6a124381f2db3ffac4c5af2a5252ac210fd7ad2

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Citations in the corpus

Targeting gut microbiota in obesity: effects of prebiotics and probiotics (2011, background): “Few studies have been aimed at finding correlations between the composition of the microbiota and the occurrence of inflammation and metabolic alterations in individuals with obesity”
Impacts of Gut Bacteria on Human Health and Diseases (2015, background): “Class Betaproteobacteria was highly increased in diabetics compared to the non-diabetic and positively correlated with plasma glucose [88].”
454‐sequencing reveals stochastic local reassembly and high disturbance tolerance within arbuscular mycorrhizal fungal communities (2012, methodology): “All analyses had samples as scaling focus, and all species data were Hellinger-transformed using the program PrCoord 1.0 (Legendre & Gallagher 2001; ter Braak & Šmilauer 2002).”
Current understanding of dysbiosis in disease in human and animal models (2016, background): “In contrast, NIDDM-associated dysbiosis is characterized by a decrease in Clostridium, an increase in Lactobacillus, and an increase in Bacteroidetes in NIDDM not associated with obesity.(31) Both IDDM and NIDDM are associated with a decrease in overall microbial diversity, including a decrease in b”
Dysbiosis of the gut microbiota in disease (2015, background): “Perhaps unsurprisingly, shifts 4 (page number not for citation purpose) Citation: Microbial Ecology in Health & Disease 2015, 26: 26191 - http://dx.doi.org/10.3402/mehd.v26.26191 in microbiota phyla have also been described in T2D (57), with metagenomics-based studies identifying discriminant metage”
Toward defining the autoimmune microbiome for type 1 diabetes (2011, result): “As described in previous studies, the ratio between Firmicutes and Bacteroidetes in human type 2 diabetes declines compared with controls (Larsen et al., 2010).”
Gut Microbiota in Cardiovascular Health and Disease (2017, background): “butyrate-producing bacteria and an increased abundance of Lactobacillus spp.(37,93,94) SCFAs, in particular butyrate, serve as energy substrates for epithelial cells of the gut.”
Germ-free C57BL/6J mice are resistant to high-fat-diet-induced insulin resistance and have altered cholesterol metabolism. (2010, background): “(6) reported that the fecal microbiota composition differs between type 2 diabetic subjects and healthy volunteers.”
Characterization of bacterial populations in Danish raw milk cheeses made with different starter cultures by denaturating gradient gel electrophoresis and pyrosequencing (2011, methodology): “…has successfully been used to monitor microbial communities in various ecosystems like sputum (Armougom et al., 2009), gut (Andersson et al., 2008; Larsen et al., 2010), soil (Acosta-Martinez, Dowd, Sun, & Allen, 2008), wounds (Dowd et al., 2008) and fermented foods (Humblot & Guyot, 2009; Roh et…”