Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand

By Blaine N. Armbruster, Xiang Li, M. Pausch, S. Herlitze, Bryan L. Roth. 2007

1,970 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology, Nutrition and Dietetics

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Citations in the corpus

Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors. (2009, positive): “When crossed with a CaMKIIa-tTA tet-driver line, tTA protein, produced when the CaMKIIa promoter is active, binds the TRE promoter to activate transcription of HA-hM3Dq; tTA binding to TRE is inhibited by doxycycline.”
The Oxytocin Receptor: From Intracellular Signaling to Behavior. (2018, background): “DREADDs are engineered GPCR, which are activated by otherwise inert small molecules (36).”
A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior (2015, positive): “Over the past several years, optogenetic and chemogenetic (Armbruster et al., 2007; Boyden et al., 2005) approaches have transformed neuroscience and other disciplines by facilitating the reversible, cell type-specific control of cellular signaling and electrical activity.”
Genetic dissection of neural circuits. (2008, positive): “Targeting of ChR2 to the soma and dendrite of genetically targeted cells will likely overcome this problem (Arnold, 2007).”
Memory engrams: Recalling the past and imagining the future (2020, background): “Artificial optogenetic or chemogenetic (72, 73) reactivation of tagged or allocated engram cells across several brain regions similarly induced memory expression without external sensory retrieval cues in a variety of tasks (42, 53, 74–81).”
DREADDs for Neuroscientists (2016, methodology): “Many labs have reported successful neuronal silencing with hM4Di, and only representative reports are cited wherein DREADDs silenced neurons (Armbruster et al., 2007), modulated amphetamine sensitization (Ferguson et al., 2011) and synaptic plasticity (Kozorovitskiy et al., 2012), regulated…”
Rapid, reversible activation of AgRP neurons drives feeding behavior in mice. (2011, methodology): “To further address whether these same neurons are required for feeding behavior, we used an identical viral approach in which we subsequently expressed the Gi/o-coupled inhibitory DREADD, designated “hM4Di”, in AgRP neurons (10, 11, 14).”