A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior
By E. Vardy, J. Robinson, Chia Li, Reid H. J. Olsen, J. Diberto, P. Giguère, Flori M. Sassano, Xi Ping Huang, Hu Zhu, D. Urban and 11 more. Neuron, 2015
355 citations recorded by the source corpus.
DREADDs are chemogenetic tools widely used to remotely control cellular signaling, neuronal activity and behavior. Here we used a structure-based approach to develop a new Gi coupled DREADD using the kappa-opioid receptor as template (KORD) that is activated by the pharmacologically inert ligand salvinorin B (SALB). Activation of virally-expressed KORD in several neuronal contexts robustly attenuated neuronal activity and modified behaviors. Additionally, co-expression of the KORD and the Gq coupled M3-DREADD within the same neuronal population facilitated the sequential and bi-directional remote control of behavior. The availability of DREADDs activated by different ligands provides enhanced opportunities for investigating diverse physiological systems using multiplexed chemogenetic actuators.
Subfields: Cellular and Molecular Neuroscience, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/5824b35cd15da129124e1f37ae2a7c7b0ef82220
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In Cellular and Molecular Neuroscience
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Citations in the corpus
- DREADDs for Neuroscientists (2016, methodology): “A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript chemogenetic activation (with hM3Dq and CNO) and inhibition (with SalB and KORD) (Vardy et al., 2015) of neuronal activity (Figure 4D).”
- The Neuroscience of Intelligence (2016, methodology): “This technique is based on creating “designer receptors exclusively activated by designer drugs,” known by the acronym DREADD (Urban & Roth, 2015); how do they find these names? Recently, researchers developed a variation of DREADD that allowed neurons to be turned on and off, rather than the previo”