Genetically encoded fluorescent indicator for intracellular hydrogen peroxide

By V. Belousov, A. Fradkov, Konstantin A. Lukyanov, D. Staroverov, K. Shakhbazov, A. Terskikh, Sergey A. Lukyanov. Nature Methods, 2006

1,225 citations recorded by the source corpus.

Subfields: Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/e5cc4c9688164293261eafab0466e5405ef667d7

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In Molecular Biology

1. H. Sies
2. C. Winterbourn
3. M. Murphy
4. B. Dickinson
5. Barry Halliwell
6. M. Jackson
7. S. Powers
8. S. Rhee

In Physiology

1. H. Sies
2. B. Dickinson
3. P. Schumacker
4. K. Davies
5. N. Larsson
6. T. Nyström
7. Linda Partridge
8. M. Jackson

Citations in the corpus

• Real-time imaging of the intracellular glutathione redox potential (2008, background): “Most recently, the H 2 O 2 -sensing properties of the bacterial protein OxyR had been exploited to develop HyPer, a yellow fluorescent protein (YFP)-based biosensor for intracellular hydrogen peroxid”
• ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis (2007, methodology): “Based on these characteristics, it is used as a faithful monitor of intracellular H 2 O 2 levels 31 .”
• Superoxide Flashes in Single Mitochondria (2008, neutral): “As would be expected of a fluorescent protein-based indicator ( Belousov et al., 2006; Nagai et al., 2001), cpYFP emission is brighter in an alkaline environment, such as that within the mitochondrial matrix (pH � 8.0, Figure S1F).”
• Exercise-Induced Oxidative Stress: Cellular Mechanisms and Impact on Muscle Force Production (2008, background): “Some new developments in this area offer promise including novel probes for specific ROS, such as HyPer, a genetically encoded probe that can be transfected into mammalian cells and in which yellow fluorescent protein is inserted into the regulatory domain of the prokaryotic hydrogen peroxide sensin”
• Fluorescent proteins and their applications in imaging living cells and tissues. (2010, methodology): “This GES design strategy resulted in a number of calcium sensors (306, 309, 322, 407, 424, 428) and was also applied to create sensors of hydrogen peroxide (39), phosphorylation (205), and membrane potential (119, 217), although the latter two directions have not yet produced effective GES.”
• Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. (2012, background): “Other approaches for monitoring intracellular H2O2 levels include the use of a redox-active biosensor, HyPer, whose design is based on OxyR, a natural bacterial H2O2 sensor [25].”
• Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics (2013, neutral): “For analytes other than Ca2+, sensors with several different colors have been constructed: Hyper, a yellow peroxide sensor (Belousov et al., 2006); blue, cyan, green, and yellow sensors for maltose (Marvin et al., 2011); a yellow sensor of molecular strain (Ichimura et al., 2012); Frex, a yellow…”
• Mitochondria-Ros Crosstalk in the Control of Cell Death and Aging (2011, neutral): “chondrial ROS production [102].”
• Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress☆ (2017, background): “The development of the HyPer probe as genetically encoded fluorescent indicator for H2O2 in intact cells and tissues was a landmark [25].”