Superoxide Flashes in Single Mitochondria
By Wang Wang, Huaqiang Fang, L. Groom, A. Cheng, Wanrui Zhang, Jie Liu, Xianhua Wang, Kaitao Li, Peidong Han, M. Zheng and 10 more. Cell, 2008
720 citations recorded by the source corpus.
SUMMARY The mitochondrion is the primary source of reactive oxygen species (ROS) in eukaryotic cells. With the aid of a novel mitochondrial matrix-targeted superoxide indicator, here we show that individual mitochondria undergo spontaneous bursts of superoxide generation, termed “superoxide flashes”. Superoxide flashes occur randomly in space and time, exhibit all-or-none properties, and reflect elementary events of superoxide production within single mitochondria across a wide diversity of cells. Individual flashes are triggered by transient openings of the mitochondrial permeability transition pore (mPTP) and are fueled by electron transfer complexes-dependent superoxide production. While decreased during cardiac hypoxia/anoxia, a flurry of superoxide flash activity contributes to the destructive rebound ROS burst observed during early reoxygenation after anoxia. The discovery of superoxide flashes reveals a novel mechanism for quantal ROS production by individual mitochondria and substantiates the central role of mPTP in oxidative stress related pathology in addition to its well-known role in apoptosis.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/87b76e60582ead74902b9b603ab1c92cb6daba4b
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In Molecular Biology
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Citations in the corpus
- Oxidant Mechanisms in Renal Injury and Disease (2016, positive): “If superoxide and ROS generation is excessive in the mitochondria, mitochondrial membrane channels (including anion channels in the inner membrane open (158) resulting in collapse of the mitochondria membrane potential and further generation of ROS by the electron transport chain (315).”
- Melatonin--a pleiotropic, orchestrating regulator molecule. (2011, background): “However, mtPTP opening should no longer be exclusively seen in connection with a fatal breakdown of DCm and apoptosis, since it has also been observed in conjunction with electron flux dynamics and the occurrence of superoxide flashes (Sheu et al., 2008; W. Wang et al., 2008).”
- Reperfusion injury and reactive oxygen species: The evolution of a concept (2015, neutral): “Similarly, endothelial cell monolayers exposed to either H/R or A/R exhibit an increased adhesivity to neutrophils (Fig.”
- Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release. (2014, methodology): “The term superoxide flashes was used to describe the RIRR in transfected cardiac myocytes (461).”