Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.

By B. Kalyanaraman, V. Darley-Usmar, K. Davies, P. Dennery, H. Forman, M. Grisham, G. Mann, K. Moore, L. Roberts, H. Ischiropoulos. Free Radical Biology & Medicine, 2012

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Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans (2017, background): “While DCFH is used in both platelets and leukocytes, dihydrorhodamine 123 (DHR123) and hydroethidine (HE) are used only in the evaluation of the oxidative burst by polymorphonuclear leukocytes (PMN) (Table 1).”
Mitochondrial ROS signaling in organismal homeostasis. (2015, background): “The use of commercially fluorescent ROS probes is widespread but oftenwithout the knowledge that these do not always readout specific ROS species faithfully and are prone to other confounding artifacts (Kalyanaraman et al., 2012).”
Quantitative detection of plasma-generated radicals in liquids by electron paramagnetic resonance spectroscopy (2013, background): “Often applied diagnostic methods for radicals in liquids are colorimetric methods including fluorescent or colorimetric dyes, but their disadvantage is that they are not specific [23].”