The Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the Brain

By Skirmantas Kriaucionis, N. Heintz. Science, 2009

2,658 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/e2eedee86aa4aa449acbdcbb19e70ab65b9e2488

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In Molecular Biology

Citations in the corpus

Comparison of sequencing-based methods to profile DNA methylation and identification of monoallelic epigenetic modifications (2010, background): “5-Hydroxymethylation of cytosine also occurs in certain human and mouse cell”
DNA methylation and cancer. (2010, background): “This kind of modification was found in cerebellar Purkinje neurons and in embryonic stem cells but the biological function of hmC and its possible impact on DNA silencing machinery is still poorly understood (Kriaucionis and Heintz, 2009).”
Epigenetic Determinants of Cancer. (2016, background): “…Gaudet et al. 2003 5-Azacytidine is FDA approved for treatment of myelodysplastic syndrome Discovery of the 5-hydroxymethylcytosine base and the TET1/2/3 enzymes that catalyze this conversion Kaminskas et al. 2005 Kriaucionis and Heintz 2009; Tahiliani et al. 2009 Adapted from You and Jones 2012.”
Global Epigenomic Reconfiguration During Mammalian Brain Development (2013, background): “The adult mammalian brain contains the highest levels of hmC that have been observed (15), accounting for about 40% of methylated CG sites in cerebellar Purkinje cells (30).”
5-hmC–mediated epigenetic dynamics during postnatal neurodevelopment and aging (2011, background): “In Purkinje cells of the cerebellum, 5-hmC is approximately 40% as abundant as 5-mC and is enriched relative to granule cell”
Dnmt1 and Dnmt3a are required for the maintenance of DNA methylation and synaptic function in adult forebrain neurons (2010, background): “More recently, it has been shown that neurons, but not non-neuronal cells, contain 5′hydroxyl-methylcytosine (5hmdC”
Cancer epigenetics: from mechanism to therapy. (2012, background): “In 2009, two seminal manuscripts describing the presence of 5-hydroxymethylcyto- sine (5hmC) offered the first insights into the metabolism of 5mC (Kriaucionis and Heintz, 2009; Tahiliani et al., 2009).”
MeCP2 binds to 5hmc enriched within active genes and accessible chromatin in the nervous system (2013, positive): “As previously reported (Doyle et al., 2008; Kriaucionis and Heintz, 2009), each of these cell types is readily visualized by the high levels of fluorescence present on translating polysomes in the cell soma.”
Rethinking how DNA Methylation Patterns are Maintained (2009, background): “However, the recent discovery of 5-hydroxymethylcytosine in mammalian DN”
Epigenetic modifications and human disease (2010, background): “2% of total nucleotides), but it seems not to be present in cancer cell line”
Role of TET enzymes in DNA methylation, development, and cancer (2016, background): “By comparison, the products of further TET-mediated oxidation, 5fC and 5caC, are observed at ∼10-fold to 100-fold lower levels than 5hmC and often close to or under the detection limit (Kriaucionis and Heintz 2009; Globisch et al. 2010; Szwagierczak et al. 2010; He et al. 2011; Ito et al. 2011; Pfaf”
Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells (2011, methodology): “c–f, Precipitation of PCR amplicons containing (1) varying amounts of 5hmC by GLIB methodology (c), anti-CMS methodology (d), or anti-5hmC antibody (e); or (2) varying amounts of 5mC by anti-5mC antibody (f).”
Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain (2011, neutral): “Notably, 5hmCs exist in relative abundance in various brain regions (Kriaucionis and Heintz, 2009).”
Tissue Distribution of 5-Hydroxymethylcytosine and Search for Active Demethylation Intermediates (2010, background): “In 2009 it was discovered that the genetic material contains aside from the nucleobases A, C, G, T, and 5-methylcytosine (mC) the additional sixth base 5-hydroxymethylcytosine (hmC) [1,2].”