BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes

By Mosè Manni, Matthew Berkeley, Mathieu Seppey, Felipe A. Simão, E. Zdobnov. Molecular biology and evolution, 2021

5,249 citations recorded by the source corpus.

Abstract Methods for evaluating the quality of genomic and metagenomic data are essential to aid genome assembly procedures and to correctly interpret the results of subsequent analyses. BUSCO estimates the completeness and redundancy of processed genomic data based on universal single-copy orthologs. Here, we present new functionalities and major improvements of the BUSCO software, as well as the renewal and expansion of the underlying data sets in sync with the OrthoDB v10 release. Among the major novelties, BUSCO now enables phylogenetic placement of the input sequence to automatically select the most appropriate BUSCO data set for the assessment, allowing the analysis of metagenome-assembled genomes of unknown origin. A newly introduced genome workflow increases the efficiency and runtimes especially on large eukaryotic genomes. BUSCO is the only tool capable of assessing both eukaryotic and prokaryotic species, and can be applied to various data types, from genome assemblies and metagenomic bins, to transcriptomes and gene sets.

Subfields: Ecology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/d7216a4c00b0f525c7e57e433d8fe0b91db9ff53

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

  1. Nikos Kyrpides
  2. Patrick S. G. Chain

In Molecular Biology

  1. Antonio Pedro Camargo
  2. Marc Feuermann
  3. Hongzhan Huang
  4. Emmanuel Boutet
  5. Nikos Kyrpides
  6. Stephen Nayfach
  7. Damien Lieberherr
  8. Aurélien Luciani

In Plant Science

  1. Marc Feuermann
  2. Nikos Kyrpides
  3. Lucille Pourcel

Citations in the corpus