Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways.

By J. C. Clemens, C. Worby, Nancy Simonson-Leff, M. Muda, T. Maehama, B. Hemmings, J. Dixon. Proceedings of the National Academy of Sciences of the United States of America, 2000

905 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d23c548ad083fe3140aaaaea500a47b5438e77ff

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Citations in the corpus

Post-transcriptional gene silencing by double-stranded RNA (2001, background): “This process is almost trivial because silencing can be achieved simply by adding the dsRNA to the culture mediu”
Drosophila Dscam is an axon guidance receptor exhibiting extraordinary molecular diversity. (2000, neutral): “S2 cells, see Clemens et al., 2000); (2)”
RNA Interference: Biology, Mechanism, and Applications (2003, methodology): “melanogaster, RNAi technology has been successfully applied to identify genes with essential roles in biochemical signaling cascades, embryonic development, and other basic cellular process (44).”
Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans (2005, background): “Pronuclear/ nuclear appearance PN and nuclei are small or missing altogether, often accompanied by spindle defects 28 (4) Nuclear pore complex, import machinery Reformation of nuclei after meiosis/mitosis T01G9.”
Analysis of cell-based RNAi screens (2006, methodology): “Subsequent studies showed that the RNAi pathway is conserved in Drosophila and vertebrates, and can be used as a tool to downregulate the expression of genes in a sequence specific manner [2,3].”
A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins. (2002, methodology): “dsRNAs of AGO2, Dmp68, or dFMR1 were introduced to S2 cells by soaking (Clemens et al. 2000).”
Regulation of TORC1 by Rag GTPases in nutrient response (2008, methodology): “Drosophila RNA interference (RNAi) experiments were performed as described previousl”
Casein Kinase Iε Modulates the Signaling Specificities of Dishevelled (2004, methodology): “The kinase assays were initiated by the addition of 2 g of GST-Jun(1-109) substrate protein, and 10 Ci of [ -32P]ATP in a volume of 25 l.”
STIM1, an essential and conserved component of store-operated Ca2+ channel function (2005, background): “Furthermore, these cells are particularly useful for gene silencing experiments, as they display highly efficient RNA interference (RNAi) with relatively simple protocols (Clemens et al., 2000; Worby et al., 2001).”
Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. (2004, background): “Double-stranded (ds) RNA induces the sequence-specific posttranscriptional gene silencing of cognate genes in numerous organisms (Cogoni and Macino 2000; Hutvagner and Zamore 2002a; Denli and Hannon 2003).”
Perspective: machines for RNAi. (2005, background): “…repeat or “hairpin” sequence, or physically introduced into cells by injection, soaking, or even feeding dsRNA-expressing bacteria (Kennerdell and Carthew 1998; Montgomery and Fire 1998; Tabara et al. 1998; Timmons and Fire 1998; Waterhouse et al. 1998; Clemens et al. 2000; Billy et al. 2001).”
Ingested double-stranded RNAs can act as species-specific insecticides. (2009, background): “An ortholog for SID-1 has not been found in any members of the order Diptera, and yet dsRNA can enter cultured Drosophila melanogaster S2 cells (Caplen et al., 2000; Clemens et al., 2000; Bettencourt-Dias and Goshima, 2009) or into tissues of fruit flies injected with dsRNA (Dzitoyeva et al., 2001).”