Low O2 tensions and the prevention of differentiation of hES cells.

By T. Ezashi, P. Das, R. Roberts. Proceedings of the National Academy of Sciences of the United States of America, 2005

850 citations recorded by the source corpus.

Subfields: Molecular Biology, Public Health, Environmental and Occupational Health

View Paper: https://www.semanticscholar.org/paper/ccef6e9214d12edcc35c963c80e5dc5f9f9a8201

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In Molecular Biology

In Public Health, Environmental and Occupational Health

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Citations in the corpus

Oxygen in stem cell biology: a critical component of the stem cell niche. (2010, background): “Interestingly, culturing under lower oxygen tensions (1%), maintains pluripotency but significantly reduces proliferation, suggesting that gradients of oxygen tensions can modulate proliferation and quiescence in stem cells (Ezashi et al., 2005).”
Hypoxia-Inducible Factors Regulate Tumorigenic Capacity of Glioma Stem Cells (2009, neutral): “Low oxygen levels promote maintenance of embryonic stem cell pluripotent potential and block differentiation (Ezashi et al., 2005).”
Hypoxia-inducible factors in stem cells and cancer (2009, neutral): “[67] demonstrated that human ES cells, when cultured either at 3–5% O2 or 21% O2 proliferate at a similar rate.”
Hypoxia enhances the generation of induced pluripotent stem cells. (2009, background): “Notably, low O2 tension promotes the survival of neural crest cells and hematopoietic stem cells and prevents differentiation of human ES cells (Danet et al., 2003; Ezashi et al., 2005; Morrison et al., 2000).”
Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated Counterparts (2011, background): “In agreement with these findings several authors have reported that hypoxia is beneficial for the maintenance of hESCs in a pluripotent state [22,23].”
Metabolic Plasticity in Stem Cell Homeostasis and Differentiation (2012, positive): “Stimulation of glycolysis in pluripotent stem cells, through hypoxia (Ezashi et al., 2005; Mohyeldin et al., 2010), inhibition of mitochondrial respiration (Varum et al., 2009), or supplementation with insulin (Chen et al., 2011), promotes stemness while inhibition of glycolysis halts proliferation…”
The role of hypoxia-inducible factors in tumorigenesis (2008, background): “In support of this notion, hypoxia has been shown to maintain human ES (hES) cells in an undifferentiated state and maintain stem cell pluripotency.(92) Interestingly, maintenance of a dedifferentiated state in hypoxic hES cells correlated with the expression of Oct4, transcription factor involved i”
Somatic oxidative bioenergetics transitions into pluripotency-dependent glycolysis to facilitate nuclear reprogramming (2011, background): “ESCs also rely on glycolytic ATP generation (Cho et al., 2006; Facucho-Oliveira et al., 2007), with pluripotency maintenance sustained under hypoxic conditions (Ezashi et al., 2005).”
Placental-related diseases of pregnancy: involvement of oxidative stress and implications in human evolution (2006, background): “Recent evidence also indicates that the hypoxia is necessary to maintain stem cells in a fully pluripotent state (Ezashi et al., 2005), for at physiological levels free radicals regulate a wide variety of cell functions, in particular transcription factors (Burton et al., 2003).”