Hypoxia enhances the generation of induced pluripotent stem cells.

By Y. Yoshida, Kazutoshi Takahashi, K. Okita, T. Ichisaka, S. Yamanaka. 2009

792 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology, Physiology

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Citations in the corpus

Somatic oxidative bioenergetics transitions into pluripotency-dependent glycolysis to facilitate nuclear reprogramming (2011, background): “Strategies that in part stimulate glycolysis, including induction of hypoxia or inhibition of the p53 pathway, increase nuclear reprogramming efficiency (Kruse and Gu, 2006; Krizhanovsky and Lowe, 2009; Yoshida et al., 2009).”
Highly efficient reprogramming to pluripotency and directed differentiation of human cells using synthetic modified mRNA (2010, methodology): “Lowoxygen (5% O2) culture conditions and a KMOS stoichiometry of 1:1:3:1 were employed, because these have been reported to promote efficient iPSC conversion (Kawamura et al., 2009; Papapetrou et al., 2009; Utikal et al., 2009; Yoshida et al., 2009).”
Nuclear reprogramming to a pluripotent state by three approaches (2010, background): “For example, exposure to a hypoxic environment or vitamin C has been shown to increase the frequency of iPS-cell generatio”
Oxygen in stem cell biology: a critical component of the stem cell niche. (2010, background): “…mechanisms and stem cell signaling that the convergence of these two pathways that was described initially in ESCs (Adelman et al., 1999), HSCs (Cipolleschi et al., 1993), NSCs (Panchision, 2009), and CSCs (Li et al., 2009) has now further been expanded to include iPSCs (Yoshida et al., 2009).”
Reprogramming of human primary somatic cells by OCT4 and chemical compounds. (2010, background): “Interestingly, a hypoxic environment and its effector, HIF-1a activation, both of which have been closely linked to promoting glycolytic metabolism, were reported to improve reprogramming efficiency for both mouse and human cells (Yoshida et al., 2009).”
The return of metabolism: biochemistry and physiology of the pentose phosphate pathway (2014, background): “Moreover, upon glycolysis-activating conditions, such as under hypoxia stimulation or after treatment with small-molecule inducers of the master metabolic regulator hypoxia-inducible transcription factor 1 (HIF-1) (see section VIII-4-d), the efficiency of somatic cell reprogramming is significantly ”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “The culturing of reprogramming cells in hypoxic conditions (Utikal et al. 2009b; Yoshida et al. 2009) or in the presence of ascorbic acid (Esteban et al. 2010) also seems to target the p53 pathway and thus may provide safer ways to boost iPSC generation.”
Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated Counterparts (2011, background): “In addition low O2 tensions have been reported to increase the reprogramming efficiency of both mouse and human somatic cells [24].”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “Accordingly, it was later described that reprogramming is more efficient under low oxygen conditions (Utikal et al., 2009; Yoshida et al., 2009).”
Hypoxic microenvironment in cancer: molecular mechanisms and therapeutic interventions (2023, background): “In addition, hypoxic conditions (<5%) could improve the efficiency of induced pluripotent stem cell (iPSC) generation from mouse and human somatic cells.(56) The hypoxic signaling microenvironment maintains stem cell selfrenewal by facilitating the reprogramming process.”
Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency (2011, positive): “…the use of other transcription factors, signaling factors, and pharmacological molecules, at least one pluripotent stem cell transcription factor—usually Oct4—is required for efficient iPSC reprogramming (Huangfu et al., 2008a, 2008b; Judson et al., 2009; Melton et al., 2010; Yoshida et al., 2009).”