Aberrant Expression of Oncogenic and Tumor-Suppressive MicroRNAs in Cervical Cancer Is Required for Cancer Cell Growth
By Xiaohong Wang, Shuāng Táng, S. Le, Robert Lu, J. Rader, C. Meyers, Zhi‐Ming Zheng. PLoS ONE, 2008
738 citations recorded by the source corpus.
MicroRNAs (miRNAs) play important roles in cancer development. By cloning and sequencing of a HPV16+ CaSki cell small RNA library, we isolated 174 miRNAs (including the novel miR-193c) which could be grouped into 46 different miRNA species, with miR-21, miR-24, miR-27a, and miR-205 being most abundant. We chose for further study 10 miRNAs according to their cloning frequency and associated their levels in 10 cervical cancer- or cervical intraepithelial neoplasia-derived cell lines. No correlation was observed between their expression with the presence or absence of an integrated or episomal HPV genome. All cell lines examined contained no detectable miR-143 and miR-145. HPV-infected cell lines expressed a different set of miRNAs when grown in organotypic raft cultured as compared to monolayer cell culture, including expression of miR-143 and miR-145. This suggests a correlation between miRNA expression and tissue differentiation. Using miRNA array analyses for age-matched normal cervix and cervical cancer tissues, in combination with northern blot verification, we identified significantly deregulated miRNAs in cervical cancer tissues, with miR-126, miR-143, and miR-145 downregulation and miR-15b, miR-16, miR-146a, and miR-155 upregulation. Functional studies showed that both miR-143 and miR-145 are suppressive to cell growth. When introduced into cell lines, miR-146a was found to promote cell proliferation. Collectively, our data indicate that downregulation of miR-143 and miR-145 and upregulation of miR-146a play a role in cervical carcinogenesis.
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/aaeab79f1b28fe0165c6efe7a40bbc613749bf7a
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Cancer Research
In Molecular Biology
Citations in the corpus
- microRNAs in cancer (2013, background): “The miR-155 gene is overexpressed in several solid tumors—for example, in breast cancer (38, 77), colon cancer (38), cervical cancer (128), pancreatic ductal adenocarcinoma (129), thyroid carcinoma (130), and lung cancer (131)—where it is considered a marker of poor prognosis.”
- miR-155 gene: a typical multifunctional microRNA. (2009, background): “miR-155 gene was found to be over expressed in several solid tumours, such as thyroid carcinoma [50], breast cancer [17, 51, 52], colon cancer [17], cervical cancer [53], pancreatic ductal adenocarcinoma (PDAC) [54, 55], and lung cancer, where it is considered to be a marker of poor prognosis [17, 5”
- MicroRNAs en route to the clinic: progress in validating and targeting microRNAs for cancer therapy (2011, neutral): “Expression levels of miR-146 family members, mir-146a and mir-146b-5p , which are encoded by separate genes, are elevated in breast, prostate, endocrine pancreatic, cervical, thyroid and ovarian carcinoma”