Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism.

By V. Tropepe, S. Hitoshi, C. Sirard, T. Mak, Janet Rossant, D. Kooy. 2001

807 citations recorded by the source corpus.

Subfields: Developmental Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c090f376b76122e88ed96475ac613aa5cb3cd8d2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Developmental Neuroscience

1. G. Keller
2. V. Tabar
3. Austin G Smith
4. Nicholas D. Socci
5. Yechiel Elkabetz
6. Georgia Panagiotakos
7. L. Studer
8. T. Jessell

In Molecular Biology

1. G. Keller
2. Qi-Long Ying
3. Austin G Smith
4. Hynek Wichterle
5. J. A. Porter
6. I. Lieberam
7. V. Tabar
8. Ali H. Brivanlou

Citations in the corpus

• Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell (2005, background): “It has also been shown that neurospheres can be obtained, albeit inefficiently, by continuous suspension culture during neural differentiation of ES cells [58].”
• Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. (2008, background): “Studies in mouse ESCs suggested the existence of FGF2/LIF-responsive primitive NSCs (Tropepe et al. 2001).”
• Directed differentiation of embryonic stem cells into motor neurons. (2002, background): “cells (Gratsch and O’Shea, 2002; Tropepe et al., 2001), but additional studies are needed to determine how Thus, the generation of a few MNs in the absence of added Hh appears to result from expression of low levels closely the early steps of neural specification in ES cells conform to pathways of n”
• β-Catenin signaling is required for neural differentiation of embryonic stem cells (2004, background): “As BMP signaling has been reported to inhibit neuronal differentiation of ES cells (Gratsch and O’Shea, 2002; Kawasaki et al., 2000; Tropepe et al., 2001) high-density RAtreated cultures were also treated with a BMP antagonist, Noggin-Fc, to determine whether BMP signaling mediated the inhibitory…”
• Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, methodology): “When cultured at low 1140 GENES & DEVELOPMENT density in serum-free medium in the presence of LIF, ES cells generate a population that has been called primitive neural stem cells (Tropepe et al. 2001).”
• Cytokines and CNS development. (2009, neutral): “Embryonic stem cells (ESCs) can give rise to cells with the characteristics of primitive neural stem cells (NSCs) in the absence of exogenous factors, but do so more efficiently in the presence of Noggin, or if BMP signaling is blocked by deletion of SMAD4, the common downstream signaling effector f”
• BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. (2003, neutral): “…in vertebrate embryos (Wilson and Hemmati-Brivanlou, 1995; Wilson undifferentiated cells and the population doubling time were slightly higher than obtained in serum plus LIFand Edlund, 2001) and have been shown to antagonize neural differentiation of ES cells (Tropepe et al., 2001; (Figure 1B).”
• Notch pathway molecules are essential for the maintenance, but not the generation, of mammalian neural stem cells. (2002, background): “Neural stem cells, which are considered the ultimate lineage precursors to all neuronal and glial cells in the mammalian nervous system, are present not only in the developing brain but also in the adult brain (Weiss et al. 1996; Gage 2000).”
• Neural induction, the default model and embryonic stem cells (2002, background): “Tropepe et al., 2001 70% cell death 82% neural colonies β-Tubulin, MAP2, Emx, Hoxb1, GFAP (astrocytes), O4 (oligodendrocytes) 30% survival + BMPs Stem like Oct4 Mouse ES cells in stromal feeder layer; + SDIA produced by the feeder layer.”