Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations

By M. J. Levene, J. Korlach, S. Turner, M. Foquet, Harold G. Craighead, W. Webb. Science, 2003

1,322 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Biophysics, Electrical and Electronic Engineering

View Paper: https://www.semanticscholar.org/paper/c03ea8d939b79146ed6174e1e20e6db986b98d45

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. Jer‐Shing Huang
2. Paolo Biagioni
3. George Papanicolaou
4. Miguel Moscoso
5. James M. Heather
6. B. Chain
7. Hanlee P. Ji
8. Anwei Chai

In Biophysics

1. Mark D. Robinson
2. Joseph D. Puglisi

In Electrical and Electronic Engineering

1. James M. Heather
2. Jer‐Shing Huang
3. B. Chain
4. Hanlee P. Ji
5. Jay Shendure
6. Olav Solgaard
7. B. Hecht
8. Paolo Biagioni

Citations in the corpus

• The sequence of sequencers: The history of sequencing DNA (2016, background): “This allows visualisation of single fl uorophore molecules close to the bottom of the ZMW, due to the zone of laser excitation being so small, even over the background of neighbouring molecules in solution [82].”
• Surface enhanced fluorescence (2008, background): “Webb and collaborators demonstrated the unique properties of metal subwavelength holes to selectively excite given fluorophores [170,171] due to the non-propagative nature of the electric field inside the holes.”
• High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence (2013, background): “Zero-mode waveguides (ZMWs) are small metallic apertures patterned on glass substrates that overcome the concentration restrictions by optically limiting background excitation (8).”
• Sequencing technologies and genome sequencing (2011, background): “Subsequently, the labeled nucleotide incorporation and detection allow us to determine the DNA sequence (Levene et al. 2003; Eid et al. 2009).”
• DNA molecules and configurations in a solid-state nanopore microscope (2003, background): “The dotted line in b represents the prediction of equation (1) in the text with t0 = 400 μs and <∆Io> = 100 pA.”
• Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures (2008, background): “The zero-mode waveguide (ZMW) is one such nanophotonic confinement structure often consisting of a circular hole in a metal cladding film on a solid transparent substrate (3).”