Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures

By J. Korlach, Patrick J. Marks, R. Cicero, J. Gray, D. Murphy, D. Roitman, Thang T. Pham, Geoff A. Otto, M. Foquet, S. Turner. 2008

245 citations recorded by the source corpus.

Subfields: Electrical and Electronic Engineering, Molecular Biology

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Citations in the corpus

High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence (2013, background): “Previous advances in nanofabrication (9), surface chemistry (10), and detection instrumentation (11) have led to ZMW-based instrumentation capable of the direct observation of DNA polymerization (12), reverse transcription (13), processive myosin motion (14), and translation by the ribosome (15, 16)”
Next generation sequencing technology: Advances and applications. (2014, background): “Instead, the fluorescent signals of the extended nucleotides are recorded in real time at 75 frames per second for the individual ZMWs.”
Real-time DNA sequencing from single polymerase molecules. (2010, methodology): “An exemplary synthesis protocol for the phospholinked dNTP Alexa Fluor 488 aminohexyl-dG5P (Korlach et al., 2008a) is given below.”
Next‐generation sequencing technologies for environmental DNA research (2012, methodology): “This platform utilizes a nano-structure called a Zero Mode Waveguide (ZMW) for real-time observation of DNA polymerization (Levene et al. 2003; Korlach et al. 2008).”
A Bioinformatician's Guide to Metagenomics (2008, background): “One next generation sequencing technology worth keeping an eye on is real time single molecule sequence determination that aims to produce multi-kilobase length reads at throughputs comparable to the short read technologies (67) and (http://visigenbio.”
Modelling and simulating generic RNA-Seq experiments with the flux simulator (2012, methodology): “In the present article we focus on several protocols employed for the currently popular Illumina and Roche 454 sequencing platforms, but the modularity of our simulation platform allows analysis of arbitrary sequencing technologies, as those announced for the future by the manufacturers Ion Torrent ”
Microbial community profiling for human microbiome projects: Tools, techniques, and challenges. (2009, methodology): “Rapid advances in sequencing technology, such as the recent availability of 400-base reads with the Titanium kit from Roche or, in the future, the availability of instruments providing 1500-base single-molecule reads, as reported by Pacific Biosciences (Korlach et al. 2008), will also improve the ac”
High‐throughput DNA sequencing – concepts and limitations (2010, background): “[18], Oxford Nanopore’s BASE technology [14] and, recently, IBM’s proposal of silicon-based nanopores [78].”