The sequence of sequencers: The history of sequencing DNA

By James M. Heather, B. Chain. Genomics, 2016

1,120 citations recorded by the source corpus.

Determining the order of nucleic acid residues in biological samples is an integral component of a wide variety of research applications. Over the last fifty years large numbers of researchers have applied themselves to the production of techniques and technologies to facilitate this feat, sequencing DNA and RNA molecules. This time-scale has witnessed tremendous changes, moving from sequencing short oligonucleotides to millions of bases, from struggling towards the deduction of the coding sequence of a single gene to rapid and widely available whole genome sequencing. This article traverses those years, iterating through the different generations of sequencing technology, highlighting some of the key discoveries, researchers, and sequences along the way.

Subfields: Biomedical Engineering, Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/1a24e75f53c684f45537806476277cb0be21156f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. Mourad Elloumi
2. Jean-François Gibrat
3. Mehdi Kchouk

In Molecular Biology

1. G. Chung
2. Mourad Elloumi
3. Jean-François Gibrat
4. Mehdi Kchouk

Citations in the corpus

• Generations of Sequencing Technologies: From First to Next Generation (2017, background): “Several previous reports and studies presented the sequencing technologies and detailed chemical mechanisms of each sequencing platform [10,11,13,15-23].”
• DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing (2018, methodology): “This method is also known as Sangers’s dideoxy sequencing method, as it uses modified bases known as dideoxy nucleotides [82].”
• Next-Generation Sequencing Technology: Current Trends and Advancements (2023, methodology): “The first attempts at sequencing DNA and RNA involved chemical degradation or enzymatic cleavage of the molecules to generate fragments that could be analyzed individually.”