DNA molecules and configurations in a solid-state nanopore microscope

By Jiali Li, Marc H. Gershow, D. Stein, E. Brandin, J. Golovchenko. Nature Materials, 2003

863 citations recorded by the source corpus.

A nanometre-scale pore in a solid-state membrane provides a new way of electronically probing the structure of single linear polymers, including those of biological interest in their native environments. Previous work with biological protein pores wide enough to let through and sense single-stranded DNA molecules demonstrates the power of using nanopores, but many future tasks and applications call for a robust solid-state pore whose nanometre-scale dimensions and properties may be selected, as one selects the lenses of a microscope. Here we demonstrate a solid-state nanopore microscope capable of observing individual molecules of double-stranded DNA and their folding behaviour. We discuss extensions of the nanopore microscope concept to alternative probing mechanisms and applications, including the study of molecular structure and sequencing.

Subfields: Biomedical Engineering, Computational Mechanics, Physical and Theoretical Chemistry

View Paper: https://www.semanticscholar.org/paper/0433608fe9286f0572e22cfc147565360ffa9ee2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. Michael Zwolak
2. M. Di Ventra
3. K. Murata
4. M. J. Kim
5. Ben Mcnally
6. Cees Dekker
7. M. Wanunu
8. Rashid Bashir

In Computational Mechanics

1. M. Di Ventra
2. Michael Zwolak
3. K. Murata
4. M. J. Kim
5. Ben Mcnally
6. Amit Meller
7. Richard B. Fair
8. Cees Dekker

In Physical and Theoretical Chemistry

1. Michael Zwolak
2. Richard B. Fair
3. M. Di Ventra

Citations in the corpus

• Digital microfluidics: is a true lab-on-a-chip possible? (2007, methodology): “These technologies include mass spectrometry (Jurinke and van den Boom 2002), nanopore sequencing (Deamer and Akeson 2000; Deamer and Branton 2002; Li and Gershow 2003), sequencing-by-hybridization (Bains and Smith 1988; Fodor et al.”
• Transport phenomena in nanofluidics (2008, neutral): “Li et al., 2003; Chen, Gu, Brandin, et al., 2004; Fologea, Gershow, Ledden, et al., 2005; Storm, Chen, Zandbergen, et al., 2005", the translocation duration of DNA !”
• Characteristics of solid-state nanometre pores fabricated using a transmission electron microscope (2007, background): “Recent studies using solid state pores have begun to emerge, demonstrating the detection of double-stranded and single-stranded DNA conformations (Li et al 2003, Heng et al 2004, Storm et al 2005a, 2005b).”
• Electrostatic Focusing of Unlabeled DNA into Nanoscale Pores using a Salt Gradient (2009, background): “We find that the funnelshaped potential V(r) near the pore leads to two capture enhancement mechanisms, evaluated in SI-1: (1) An exponential attempt rate enhancement, resulting from the fact that the potential well V(r) traps the DNA a distance rg from the pore mouth, where threading is repeatedly ”
• Advanced sequencing technologies: methods and goals (2004, neutral): “A creative single-molecule approach that is unlike all of the above methods is nanopore sequencing, which is being developed by Agilent, and by the Branton and Deamer group”
• Nanopore sensors for nucleic acid analysis. (2011, background): “, analogous to the DNA-induced current blockades seen in SiN nanopore”