High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence

By Jin Chen, Ravindra V. Dalal, A. Petrov, Albert Tsai, S. O’Leary, Karen Chapin, Janice Cheng, Mark Ewan, P. Hsiung, P. Lundquist and 3 more. 2013

138 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

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In Biophysics

1. Joseph D. Puglisi

In Molecular Biology

1. Jinfan Wang
2. I. S. Fernández
3. Joseph D. Puglisi
4. Christopher P. Lapointe
5. Alex G. Johnson
6. Rosslyn Grosely

Citations in the corpus

• RACK1 on and off the ribosome (2019, methodology): “We applied a zero-mode waveguide-based (ZMW) system to make real-time smFRET measurements (Chen et al. 2014), and selected the IRES(C44) label since it had higher FRET efficiency with RACK1-ybbR in comparison to IRES(U56).”
• Mechanisms that ensure speed and fidelity in eukaryotic translation termination (2021, methodology): “We first prepared 80S ribosomal complexes programmed on 5’-biotinylated M-Stop mRNAs with Cy5-labeled 60S subunits; these complexes were tethered to neutravidin-coated Zero-Mode Waveguide (ZMW) surfaces (15, 16).”
• Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation (2020, methodology): “All real-time imaging was conducted using a modified Pacific Biosciences RSII DNA sequencer (35).”