Sequencing technologies and genome sequencing

By C. Pareek, Rafał Smoczyński, A. Tretyn. Journal of Applied Genetics, 2011

815 citations recorded by the source corpus.

The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. In the relatively short time frame since 2005, the HT-NGS technologies are revolutionizing the human and animal genome researches by analysis of chromatin immunoprecipitation coupled to DNA microarray (ChIP-chip) or sequencing (ChIP-seq), RNA sequencing (RNA-seq), whole genome genotyping, genome wide structural variation, de novo assembling and re-assembling of genome, mutation detection and carrier screening, detection of inherited disorders and complex human diseases, DNA library preparation, paired ends and genomic captures, sequencing of mitochondrial genome and personal genomics. In this review, we addressed the important features of HT-NGS like, first generation DNA sequencers, birth of HT-NGS, second generation HT-NGS platforms, third generation HT-NGS platforms: including single molecule Heliscope™, SMRT™ and RNAP sequencers, Nanopore, Archon Genomics X PRIZE foundation, comparison of second and third HT-NGS platforms, applications, advances and future perspectives of sequencing technologies on human and animal genome research.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/0b90caf141ec80ce2bbab6a10ba9bcb16738e45f

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Citations in the corpus

Generations of Sequencing Technologies: From First to Next Generation (2017, methodology): “We distinguish the second generation sequencing technologies which refer to the newest sequencing technologies developed in the NGS environment after the first generation [11,12], they are characterized by the need to prepare amplified sequencing banks before starting the sequencing of amplified DNA”
SNP Discovery through Next-Generation Sequencing and Its Applications (2012, methodology): “Heliscope sequencers can generate up to 28 GB of sequence data per run (50 channels) with maximum read length of 55 bp at ∼ 99% accuracy [31].”
The sequence of sequencers: The history of sequencing DNA (2016, background): “There is considerable discussion about what de ﬁ nes the different generations of DNA sequencing technology, particularly regarding the division from second to third [73,74,75,76].”