A DNA vector-based RNAi technology to suppress gene expression in mammalian cells

By Guangchao Sui, Christina Y Soohoo, E. Affar, F. Gay, Yang Shi, W. Forrester. Proceedings of the National Academy of Sciences of the United States of America, 2002

1,182 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/bf591969644d1f26270cc19dcb3530464171339f

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Citations in the corpus

Lentivirus-delivered stable gene silencing by RNAi in primary cells. (2003, background): “Capable of activating naive B and T cells, they play an important role in initiating host immune responses ( Steinman and Pope 2002 ).”
Antisense technologies. Improvement through novel chemical modifications. (2003, methodology): “Short hairpin RNA was transcribed using RNA polymerase III promotors that normally control the transcription of either the small nuclear RNA U6 [118,119,121,122] or the H1 RNA component of RNase P [123].”
The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases. (2007, positive): “To determine the function of endogenous Smcx in rodent neuronal morphogenesis, we used a plasmid-based method of RNAi ( Sui et al., 2002 ) to induce the knockdown of Smcx in primary granule neurons cultured from postnatal day 6 rat (P6) pups.”
Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. (2004, background): “LSD1 occupancy at the above promoters ( 1 to 2 kb) (Figure 7C) was shown by chromatin immunoprecipitation (ChIP) in the lsd1 and control RNAi-treated HeLa cells (panel D3).”
Coordinated histone modifications mediated by a CtBP co-repressor complex (2003, methodology): “We used the DNA vector-based RNA-mediated interference (RNAi) approac”
GA binding protein regulates interleukin 7 receptor α-chain gene expression in T cells (2004, methodology): “Silencing GABP reduces Il7r expression We next used a vector based on the U6 promoter and RNA polymerase III (pBS/U6”
mTOR Controls Cell Cycle Progression through Its Cell Growth Effectors S6K1 and 4E-BP1/Eukaryotic Translation Initiation Factor 4E (2004, methodology): “(52) between the ApaI and EcoRI restriction sites to express hairpin RNA from”
SIRT1 deacetylase protects against neurodegeneration in models for Alzheimer's disease and amyotrophic lateral sclerosis (2007, methodology): “P53 RNAi sequence were selected based on the criteria proposed by Sui et al (2002).”
RNAi therapeutics: principles, prospects and challenges. (2007, background): “Soon after the realization that synthetic siRNA could be introduced into mammalian cells and trigger RNAi without activating type 1 interferon responses, researchers discovered that DNAbased expression cassettes could be generated that express short hairpins (shRNA) or separate sense and antisense 2”