Lentivirus-delivered stable gene silencing by RNAi in primary cells.

By S. Stewart, Derek M. Dykxhoorn, D. Palliser, H. Mizuno, E. Yu, D. An, David M. Sabatini, I. Chen, William C. Hahn, Phillip A. Sharp and 2 more. RNA: A publication of the RNA Society, 2003

1,579 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/906a8c0b8ca84373460c0b9b15a0ab216a0b6ca2

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Citations in the corpus

Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. (2008, methodology): “Plasmids. Sequences targeting the E-cadherin and h-catenin shRNAs were taken from the website of the RNAi consortium at the Broad institute and cloned into the PLKO-puro and PLKO-hygro vectors6 ( 26 ).”
Killing the messenger: short RNAs that silence gene expression (2003, background): “To overcome some of these limitations, several groups have reported the use of retrovirus vectors to deliver siRNAs into cell”
Early dissemination seeds metastasis in breast cancer (2016, positive): “Retroviral delivery of transgenes was performed as described previously 44 .”
Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules (2018, neutral): “…al., 1998) Addgene #12253 Plasmid: pMDLg/pRRE Gift from Didier Trono (Dull et al., 1998) Addgene #12251 Plasmid: pCMV-VSV-G Gift from Bob Weinberg (Stewart et al., 2003) Addgene #8454 Software and Algorithms Enrichr Gene Set Enrichment Analysis (Kuleshov et al., 2016)…”
Telomerase maintains telomere structure in normal human cells. (2003, positive): “By immunizing mice with a recombinant version of the et al., 2003; Stewart et al., 2003a).”
Genomic positional conservation identifies topological anchor point (tap)RNAs linked to developmental loci (2016, methodology): “Briefly, HPLC-purified oligomers were annealed using final concentrations of 3μM each in 1xNEB buffer 2 and used in ligations into pLKO vectors digested with AgeI and EcoRI (pLKO.1 puro (addgene #8453; (Stewart et al., 2003)) and Tet-pLKO-puro (addgene #21915; (Wiederschain et al., 2009)).”
Inhibiting DNA methylation causes an interferon response in cancer via dsRNA including endogenous retroviruses (2015, positive): “TLR3, MAVS, and STING shRNA were performed according to established methods (Stewart et al., 2003).”
The epithelial-mesenchymal transition generates cells with properties of stem cells. (2008, methodology): “The pWZL-Blast-Snail-ER and PWZL-Blast-Twist-ER constructs were generated by replacing the MYC cDNA of pWZL-Blast-DN-MycER ( Littlewood et al., 1995; Watnick et al., 2003) with the hSnail cDNA PCR amplified from pBp-hSnail or the mTwist cDNA PCR-amplified from pBpmTwist.”
Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors (2005, methodology): “HeLa cells stably expressing EGFP (HeLa-GFP) were previously describe”