Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells

By Alexander Meissner, Marius Wernig, R. Jaenisch. Nature Biotechnology, 2007

796 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/b397f2c740f9a8daefed33bc992d1905d4c7f5c3

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Citations in the corpus

Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition (2008, background): “iPS cells generated in this way arise at low frequency and take 3 wk or longer to emerge [14,26].”
Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease (2008, methodology): “To test the neural differentiation potential of reprogrammed fibroblasts in vitro, we chose the Nanog-selected iPS cell lines N8, N10, and N14, the Oct4-selected iPS cell lines O9 and O18 (11), as well as the non-drug-selected iPS cell line OG-14 (12), and applied a multistage differentiation protoc”
A Small Molecule Inhibitor of Tgf-β Signaling Replaces Sox2 in Reprogramming by Inducing Nanog (2009, positive): “Activation of an Oct4∷ GFP reporter gene in colonies with an ES cell morphology has been shown to be a stringent assay for reprogramming (Meissner et al., 2007).”
Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency. (2008, positive): “Inducible Expression of Reprogramming Factors in the B Cell Lineage Our initial strategy was to determine whether Oct4, Sox2, Klf4 and c-Myc transcription factors, which were shown to be sufficient to reprogram mouse and human fibroblast cultures (Meissner et al., 2007; Okita et al., 2007; Takahashi”
Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. (2008, background): “, 2006) and a GFP allele driven by either the endogenous Oct4 or Nanog promoter (Maherali et al., 2007; Meissner et al., 2007).”
A combined chemical and genetic approach for the generation of induced pluripotent stem cells. (2008, positive): “…human somatic cells into induced pluripotent stem (iPS) cells have opened up tremendous opportunities for the stem cell field (Maherali et al., 2007; Meissner et al., 2007; Nakagawa et al., 2008; Okita et al., 2007; Takahashi et al., 2007; Takahashi and Yamanaka, 2006; Wernig et al., 2007, 2008;…”
c-Myc is dispensable for direct reprogramming of mouse fibroblasts. (2008, background): “…to initiate a reprogramming process that results in the transformation of mouse fibroblasts into embryonic stem (ES)-like cells designated as induced pluripotent stem (iPS) cells (Maherali et al., 2007; Meissner et al., 2007; Okita et al., 2007; Takahashi and Yamanaka, 2006; Wernig et al., 2007).”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, methodology): “Importantly, several studies report the isolation of high quality iPS cells without drug selection, but by simply using morphological criteria (Blelloch et al., 2007; Meissner et al., 2007).”
Mechanical control of stem cell differentiation (2008, background): “Given the poor efficiency of forming induced pluripotent cells in vitro (Meissner et al., 2007; Takahashi et al., 2007) and the apparent importance of microenvironmental cues in forming “stem cell niches” (Lensch et al.”
Oct4-induced pluripotency in adult neural stem cells. (2009, positive): “Initially, generation of pluripotent cells was achieved by ectopic expression of four factors (Oct4, Sox2, Klf4, and c-Myc) from mouse and human somatic cells (Lowry et al., 2008; Maherali et al., 2007; Meissner et al., 2007; Okita et al., 2007, 2008; Park et al., 2008; Stadtfeld et al., 2008; Takah”
Role of the murine reprogramming factors in the induction of pluripotency (2009, positive): “While Thy1 downregulation occurs in the majority of cells, gain of SSEA1 and activation of pluripotency regulators happens with very low efficiency, suggesting that as yet unknown epigenetic barriers need to be overcome to induce pluripotency (Huangfu et al., 2008; Meissner et al., 2007; Mikkelsen e”