Microarray-based, high-throughput gene expression profiling of microRNAs

By P. Nelson, D. A. Baldwin, L. M. Scearce, J. Carl Oberholtzer, John W. Tobias, Zissimos Mourelatos, Blvd, Penn Microarray, Penn. Nature Methods, 2004

627 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b0a8fe1d6112313e68f8e3a716a9221dc29cfacd

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Citations in the corpus

Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues. (2008, background): “Although the small size of miRNA (17–25 nucleotides [nt]) creates challenges for their detection, recent innovative adaptations of existing technologies for gene expression profiling, such as microarrays and quantitative RT-PCR (qRT-PCR), have emerged that make large-scale characterizations of miRNA”
Micro-RNA speciation in fetal, adult and Alzheimer's disease hippocampus (2007, methodology): “1) [2,3] and human 5S ribosomal RNA (5S rRNA) were spotted onto GeneScreen Plus nylon membranes using a Biomek 2000 laboratory automation workstation (Beckmann, Fullerton, California, USA) and were crosslinked, baked, hybridized and probed according to the manufacturer’s protocol (NEN Research Produ”
Genomics of microRNA. (2006, methodology): “RAKE assay Mature miRNA CC CCC Human HeLa, Jurkat, malignant meningioma, oligodendroglioma [ 92 ]”
MicroRNA miR-21 overexpression in human breast cancer is associated with advanced clinical stage, lymph node metastasis and patient poor prognosis. (2008, background): “An article, published in Nature Methods first reported that miRNAs could be isolated and profiled from FFPE tissue ( Nelson et al. 2004 ).”
A novel and universal method for microRNA RT-qPCR data normalization (2009, background): “Despite the small size of miRNA molecules, several technologies have been developed that enable high-throughput and sensitive miRNA profiling, such as microarrays [3-8], real-time quantitative PCR (RT-qPCR) [9,10] and bead-based flow cytometry [2].”
Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. (2005, background): “Other systems have recently been described for the expression profiling of miRNAs (Babak et al. 2004; Liu et al. 2004; Miska et al. 2004; Nelson et al. 2004; Thomson et al. 2004).”
microRNAs as oncogenes and tumor suppressors. (2007, positive): “Several laboratories have modified DNA microarray technology to form miRNA microarray technology (Babak et al., 2004; Barad et al., 2004; Liang et al., 2005; Liu et al., 2004; Nelson et al., 2004a,b; Thomson et al., 2004).”
MicroRNA signatures in human cancers (2006, background): “— or the high-throughput array-based Klenow enzyme (RAKE) assa”