Amino acids mediate mTOR/raptor signaling through activation of class 3 phosphatidylinositol 3OH-kinase.

By T. Nobukuni, M. Joaquin, M. Roccio, Stephen G Dann, S. Kim, P. Gulati, Maya P Byfield, J. Backer, F. Natt, J. Bos and 2 more. Proceedings of the National Academy of Sciences of the United States of America, 2005

830 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/af087846bdec44e584b8bf984a81b0386fe9c264

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

mTOR signaling at a glance (2009, background): “By contrast, the phosphorylation state of TSC2 and GSK3 is not affected by mTORC2 inactivation.”
The PTEN–PI3K pathway: of feedbacks and cross-talks (2008, background): “In an elegant study, they found that the ability of mTORC1 to sense amino acid was not dependent on the class 1 PI3K, but on the class 3 (Nobukuni et al., 2005).”
Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG (2006, methodology): “PI(K)C3 (hVps34) kinase assay was performed in vitro as previously describe”
Metformin, Independent of AMPK, Inhibits mTORC1 In a Rag GTPase-Dependent Manner (2010, background): “Nutrients, unlike insulin, have been shown to mediate mTORC1 signaling through class 3 PI3K, hVps34 (Byfield et al., 2005; Nobukuni et al., 2005), and, more recently, Rag GTPases (Kim et al.”
Regulation Mechanisms and Signaling Pathways of Autophagy (2009, background): “Other studies indicate that amino acids also activate mTOR via class III PtdIns3K (hVps34) (10,102) (Figure 2a).”
ULK1 induces autophagy by phosphorylating Beclin-1 and activating Vps34 lipid kinase (2013, methodology): “Active GST-ULK1 (1-649) and GST-ULK2 (1-478) from insect cells was purchased from CQuential Solutions (Moraga, CA).”
Autophagy: process and function. (2007, background): “One candidate sensor is GCN2, a tRNAbinding protein kinase (Tallóczy et al. 2002), and recent reports suggest the presence of other amino acid signaling pathways involving class III phosphatidylinositol 3 (PI3)-kinase and Beclin 1 (Byfield et al. 2005; Nobukuni et al. 2005).”
Phosphatidylinositol 5-phosphate 4-kinase (PIP4K) regulates TOR signaling and cell growth during Drosophila development (2013, background): “Likewise the generation of phosphatidylinositol 3-phosphate (PI3P) via class III PI3K/vacuolar protein sorting 34 (Vps34) is reported to be a key signaling input into TOR activity in mammalian cells (6).”
TOR signaling in growth and metabolism. (2006, background): “Recent studies suggest that hVPS34, a class III PI3K (converts phosphatidylinositol to phosphatidylinositol-3-phosphate), signals amino acid availability to mTORC1 independently of the TSC1-TSC2/Rheb axis (Byfield et al., 2005; Nobukuni et al., 2005).”
Dietary protein source significantly alters growth performance, plasma variables and hepatic gene expression in rainbow trout (Oncorhynchus mykiss) fed amino acid balanced diets (2012, background): “The molecular mechanism of this molecule seems to regulate the assembly of the Tuberous sclerosis complex (TSC), described as a down regulator of the activity of the mTOR cascade (Kimball et al., 2008; Nobukuni et al., 2005).”